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Literature summary for 2.5.1.16 extracted from

  • Sindhu, R.K.; Cohen, S.S.
    Putrescine aminopropyltransferase (spermidine synthase) of Chinese Cabbage (1983), Methods Enzymol., 94, 279-285.
No PubMed abstract available

General Stability

General Stability Organism
dialysis against glycine-NaOH buffer, pH 8.8 inactivates Brassica rapa subsp. pekinensis
DTT and/or EDTA do not stabilize Brassica rapa subsp. pekinensis
heat-inactivated enzyme preparation does not restore the activity Brassica rapa subsp. pekinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
gel filtration Brassica rapa subsp. pekinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Escherichia coli
-
5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Brassica rapa subsp. pekinensis
-
5'-methylthioadenosine + spermidine
-
?

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. pekinensis
-
Chinese cabbage, var Pak Choy
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin and ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified Brassica rapa subsp. pekinensis

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine first discovered Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Brassica rapa subsp. pekinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.28
-
-
Brassica rapa subsp. pekinensis

Storage Stability

Storage Stability Organism
-15°C, disrupted protoplasts lose more than 90% of activity within 1 week Brassica rapa subsp. pekinensis
-20°C, about 30% loss of activity in the final enzyme preparation within 1 month Brassica rapa subsp. pekinensis
-20ºC, stable after the acetone precipitation Brassica rapa subsp. pekinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Escherichia coli 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Escherichia coli 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermidine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Brassica rapa subsp. pekinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
at a given pH, the activity of the enzyme in Tris buffer is lower than in phosphate or Gly-NaOH buffers Brassica rapa subsp. pekinensis
8.8
-
in Gly-NaOH buffer Brassica rapa subsp. pekinensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
no activity detected in phosphate buffer Brassica rapa subsp. pekinensis
10
-
no activity detected in Gly-NaOH buffer Brassica rapa subsp. pekinensis