Any feedback?
Literature summary for 2.5.1.16 extracted from
- Putrescine aminopropyltransferase (Escherichia coli) (1983), Methods Enzymol., 94, 265-270.
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing inactivates | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-ethylthioadenosine | - |
Escherichia coli |  |
| additional information | inhibition by end products of reaction; no inhibition by carbonyl binding reagents | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
| spermidine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 8.2, 37ºC, spectrophotometric assay | Escherichia coli | |
| 0.012 | - |
1,4-diaminobutane | pH 8.2, 37ºC, spectrophotometric assay | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, sedimentation equilibrium centrifugation and SDS-PAGE | Escherichia coli |
| 72000 | - |
W strain | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extraction, ammonium sulfate fractionation, chromatography on calcium phosphate cellulose, DEAE-Sephadex, chromatography on hydroxyapatite, disc gel electrophoresis | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.83 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, purified enzyme, 10-20% loss of activity within 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine | at a reduced rate | Escherichia coli | 5'-methylthioadenosine + spermine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
| S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Escherichia coli | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, sedimentation equilibrium centrifugation and SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
- |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
