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Literature summary for 2.5.1.16 extracted from

  • Lee, M.J.; Yang, Y.T.; Lin, V.; Huang, H.
    Site-directed mutations of the gatekeeping loop region affect the activity of Escherichia coli spermidine synthase (2013), Mol. Biotechnol., 54, 572-580.
    View publication on PubMed
Search for more literature for 2.5.1.16

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain SG13009 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C159A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
D158A site-directed mutagenesis, inactive mutant Escherichia coli
D161A site-directed mutagenesis, inactive mutant Escherichia coli
I163A site-directed mutagenesis, replacement of Ile163 has no influence on EcSDPS activity Escherichia coli
P162A site-directed mutagenesis, replacement of Pro162 has no influence on EcSDPS activity Escherichia coli
P165Q site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli
T160A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant I163A Escherichia coli
0.032
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant wild-type enzyme Escherichia coli
0.033
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant C159S Escherichia coli
0.035
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P162A Escherichia coli
0.038
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant C159A Escherichia coli
0.05
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant T160A Escherichia coli
0.059
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P165A Escherichia coli
0.0778
-
 putrescine pH 7.5, 37°C, recombinant wild-type enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33252
-
 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation Escherichia coli
33823
-
 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation Escherichia coli
36500
-
 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosylmethioninamine + putrescine Escherichia coli
-
 S-methyl-5'-thioadenosine + spermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P09158 strain MG1655, gene speE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain SG13009 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine Escherichia coli SPDS follows the ping-pong mechanism, in which an aminopropylated enzyme intermediate was formed by the interaction of SPDS with dcSAM, prior to reaction with putrescine Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosylmethioninamine + putrescine
-
 Escherichia coli S-methyl-5'-thioadenosine + spermidine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 36500, 2 * 33823, recombinant His6-tagged enzyme, mass spectrometry, 2 * 33252, His6-tagged enzyme, sequence calculation Escherichia coli

Synonyms

Synonyms Comment Organism
SPDS
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50 sharp drop in activity above optimal temperature 50°C, profile overview Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.128
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant C159A Escherichia coli
0.135
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant T160A Escherichia coli
0.138
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant I163A Escherichia coli
0.14
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant wild-type enzyme Escherichia coli
0.145
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant C159S Escherichia coli
0.15
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P165A Escherichia coli
0.156
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P162A Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function spermidine synthase plays a crucial role in cell proliferation and differentiation Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P165A Escherichia coli
2.7
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant T160A Escherichia coli
3.4
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant C159A Escherichia coli
4.4
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant wild-type enzyme and SPS mutant C159S Escherichia coli
4.5
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant P162A Escherichia coli
4.8
-
 S-adenosylmethioninamine pH 7.5, 37°C, recombinant SPS mutant I163A Escherichia coli