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Literature summary for 2.5.1.18 extracted from

  • Asaoka, K.
    Affinity purification and characterization of glutathione S-transferases from bovine liver (1984), J. Biochem., 95, 685-696.
    View publication on PubMed

General Stability

General Stability Organism
glycerol, 30%, protects against thermal inactivation Bos taurus
sucrose, 0.5 M, protects against thermal inactivation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
butanol 50% inactivation at 5% Bos taurus
Cd2+
-
Bos taurus
Co2+ weak Bos taurus
Cu2+
-
Bos taurus
Hg2+
-
Bos taurus
Ni2+ weak Bos taurus
Zn2+
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
glutathione isozyme I Bos taurus
0.16
-
glutathione isozyme II Bos taurus
0.55
-
1-chloro-2,4-dinitrobenzene isozyme I Bos taurus
1.3
-
3,4-dinitrobenzoic acid isozymes I and II Bos taurus
2
-
1,2-dinitrobenzene isozymes I and II Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2 * 27000, SDS-PAGE Bos taurus
49000
-
gel filtration Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 25% acetone: 70-80% inactivation Bos taurus
Acetone 5% acetone: stable Bos taurus
Butanol stable at 5% butanol Bos taurus
dimethyl sulfoxide stable at 5% and 25% dimethyl sulfoxide Bos taurus
dimethylformamide 5% dimethylformamide: stable, 25% dimethylformamide: 70-80% inactivation Bos taurus
dioxane 5% dioxane: stable Bos taurus
dioxane 25% dioxane: 70-80% inactivation Bos taurus
Ethanol 25% ethanol: 70-80% inactivation Bos taurus
Ethanol 5% ethanol: stable Bos taurus
Methanol 25% methanol: 70-80% inactivation Bos taurus
Methanol 5% methanol: stable Bos taurus
methyl cellosolve stable at 5% and 25% methyl cellosolve for 2 h Bos taurus
propanol 25% propanol: 70-80% inactivation Bos taurus
propanol 5% propanol: stable Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
liver: 2 major forms: I and II
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
with 1,2-dinitrobenzene Bos taurus
15
-
with 1-chloro-2,4-dinitrobenzene Bos taurus

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 18 h Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dinitrobenzene
-
Bos taurus ?
-
?
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
Bos taurus ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
-
Bos taurus S-2,4-dinitrophenylglutathione + HCl
-
?
glutathione + 3,4-dinitrobenzoic acid
-
Bos taurus ?
-
?
glutathione + 5-nitrofurfural
-
Bos taurus ?
-
?
glutathione + 5-nitrofurfural diacetal
-
Bos taurus ?
-
?
additional information specificity Bos taurus ?
-
?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Bos taurus HX + R-S-glutathione
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
3 h, stable Bos taurus
35
-
1 h, stable Bos taurus
37
-
fairly stable between pH 6 and 10 Bos taurus
40
-
10 min, stable Bos taurus
55
-
above, 10 min, 50% inactivation Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6: about 40% of activity maximum, pH 8: about 55% of activity maximum Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
10 min, 37°C, 50% loss of activity, 18 h at 4°C, 50% loss of activity Bos taurus
6 10 10 min at 37°C, stable, 18 h at 4°C, stable Bos taurus