General Stability | Organism |
---|---|
glycerol, 30%, protects against thermal inactivation | Bos taurus |
sucrose, 0.5 M, protects against thermal inactivation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
butanol | 50% inactivation at 5% | Bos taurus | |
Cd2+ | - |
Bos taurus | |
Co2+ | weak | Bos taurus | |
Cu2+ | - |
Bos taurus | |
Hg2+ | - |
Bos taurus | |
Ni2+ | weak | Bos taurus | |
Zn2+ | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
glutathione | isozyme I | Bos taurus | |
0.16 | - |
glutathione | isozyme II | Bos taurus | |
0.55 | - |
1-chloro-2,4-dinitrobenzene | isozyme I | Bos taurus | |
1.3 | - |
3,4-dinitrobenzoic acid | isozymes I and II | Bos taurus | |
2 | - |
1,2-dinitrobenzene | isozymes I and II | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Bos taurus |
49000 | - |
gel filtration | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 25% acetone: 70-80% inactivation | Bos taurus |
Acetone | 5% acetone: stable | Bos taurus |
Butanol | stable at 5% butanol | Bos taurus |
dimethyl sulfoxide | stable at 5% and 25% dimethyl sulfoxide | Bos taurus |
dimethylformamide | 5% dimethylformamide: stable, 25% dimethylformamide: 70-80% inactivation | Bos taurus |
dioxane | 5% dioxane: stable | Bos taurus |
dioxane | 25% dioxane: 70-80% inactivation | Bos taurus |
Ethanol | 25% ethanol: 70-80% inactivation | Bos taurus |
Ethanol | 5% ethanol: stable | Bos taurus |
Methanol | 25% methanol: 70-80% inactivation | Bos taurus |
Methanol | 5% methanol: stable | Bos taurus |
methyl cellosolve | stable at 5% and 25% methyl cellosolve for 2 h | Bos taurus |
propanol | 25% propanol: 70-80% inactivation | Bos taurus |
propanol | 5% propanol: stable | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
liver: 2 major forms: I and II | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
with 1,2-dinitrobenzene | Bos taurus |
15 | - |
with 1-chloro-2,4-dinitrobenzene | Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity after 18 h | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1,2-dinitrobenzene | - |
Bos taurus | ? | - |
? | |
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane | - |
Bos taurus | ? | - |
? | |
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Bos taurus | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
glutathione + 3,4-dinitrobenzoic acid | - |
Bos taurus | ? | - |
? | |
glutathione + 5-nitrofurfural | - |
Bos taurus | ? | - |
? | |
glutathione + 5-nitrofurfural diacetal | - |
Bos taurus | ? | - |
? | |
additional information | specificity | Bos taurus | ? | - |
? | |
RX + glutathione | RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange | Bos taurus | HX + R-S-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
3 h, stable | Bos taurus |
35 | - |
1 h, stable | Bos taurus |
37 | - |
fairly stable between pH 6 and 10 | Bos taurus |
40 | - |
10 min, stable | Bos taurus |
55 | - |
above, 10 min, 50% inactivation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6: about 40% of activity maximum, pH 8: about 55% of activity maximum | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
10 min, 37°C, 50% loss of activity, 18 h at 4°C, 50% loss of activity | Bos taurus |
6 | 10 | 10 min at 37°C, stable, 18 h at 4°C, stable | Bos taurus |