Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Wu, S.; Dou, W.; Wu, J.J.; Wang, J.J.
    Purification and partial characterization of glutathione S-transferase from insecticide-resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae) (2009), Arch. Insect Biochem. Physiol., 70, 136-150.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dichlorvos the enzymes from different populations show different susceptibilities to dichlorvos Liposcelis paeta

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Liposcelis paeta
Ca2+
-
Liposcelis paeta
carbosulfan in vivo inhibition Liposcelis paeta
chlorpyrifos in vivo inhibition Liposcelis paeta
cypermethrin in vivo inhibition Liposcelis paeta
Ethacrynic acid specific inhibition Liposcelis paeta
Hg2+
-
Liposcelis paeta
Mg2+
-
Liposcelis paeta
Mn2+
-
Liposcelis paeta
additional information the three populations of Liposcelis paeta show different susceptibilities to different insecticides, even after they are reared on diets consisting of 25% ethacrynic acid, no inhibition by Ba2+ Liposcelis paeta
Zn2+
-
Liposcelis paeta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information purified enzyme, Michaelis-Menten kinetic analysis, overview Liposcelis paeta
0.68
-
glutathione pH 7.5, 37°C, enzyme of Wuzhou population Liposcelis paeta
0.71
-
glutathione pH 7.5, 37°C, enzymes of Hezhou and Nanyang population Liposcelis paeta
6.4
-
1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Nanyang population Liposcelis paeta
16
-
1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Wuzhou population Liposcelis paeta
223.6
-
1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Hezhou population Liposcelis paeta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19420
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
20130
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
20430
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
23310
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
50790
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
53140
-
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene Liposcelis paeta the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Organism

Organism UniProt Comment Textmining
Liposcelis paeta
-
three field populations collected from Nanyang city of Henan Province, and Wuzhou and Hezhou cities of Guangxi Province, China
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by glutathione affinity chromatography, three field populations from Nanyang, purification 42.5fold, Wuzhou, purification 64.5fold, and Hezhou purification 67.4fold Liposcelis paeta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00065
-
crude enzyme of Wuzhou population Liposcelis paeta
0.0012
-
crude enzyme of Nanyang population Liposcelis paeta
0.0021
-
crude enzyme of Hezhou population Liposcelis paeta
0.0494
-
purified enzyme of Nanyang population Liposcelis paeta
0.0645
-
purified enzyme of Wuzhou population Liposcelis paeta
0.0674
-
purified enzyme of Hezhou population Liposcelis paeta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene Liposcelis paeta S-(2,4-dinitrophenyl)glutathione + HCl
-
?
additional information no activity with 1,2-dichloro-nitrobenzene Liposcelis paeta ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Liposcelis paeta
GST
-
Liposcelis paeta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
enzyme from Nanyang population Liposcelis paeta
40
-
enzymes from Wuzhou population and from Hezhou population Liposcelis paeta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 42 temperature profiles of enzyme from different populations, overview Liposcelis paeta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
enzyme from Nanyang population Liposcelis paeta
7.5 8 enzyme from Wuzhou population Liposcelis paeta
8
-
enzyme from Hezhou population Liposcelis paeta

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH profiles of enzyme from different populations, overview Liposcelis paeta

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
IC50 values for insecticides and metal ions for the enzymes from different populations, overview Liposcelis paeta additional information
0.000000395 0.000000842
-
Liposcelis paeta Zn2+
0.000000873 0.00000585
-
Liposcelis paeta Hg2+
0.00000181 0.00000203
-
Liposcelis paeta Ethacrynic acid
0.000243 0.000583
-
Liposcelis paeta Mg2+
0.00039 0.000978
-
Liposcelis paeta cypermethrin
0.00132 0.00512
-
Liposcelis paeta Mn2+
0.00158 0.0284
-
Liposcelis paeta Ca2+
0.0056 0.0148 pH 7.5, 37°C Liposcelis paeta chlorpyrifos
0.055 0.806
-
Liposcelis paeta carbosulfan