Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dichlorvos | the enzymes from different populations show different susceptibilities to dichlorvos | Liposcelis paeta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Liposcelis paeta | |
Ca2+ | - |
Liposcelis paeta | |
carbosulfan | in vivo inhibition | Liposcelis paeta | |
chlorpyrifos | in vivo inhibition | Liposcelis paeta | |
cypermethrin | in vivo inhibition | Liposcelis paeta | |
Ethacrynic acid | specific inhibition | Liposcelis paeta | |
Hg2+ | - |
Liposcelis paeta | |
Mg2+ | - |
Liposcelis paeta | |
Mn2+ | - |
Liposcelis paeta | |
additional information | the three populations of Liposcelis paeta show different susceptibilities to different insecticides, even after they are reared on diets consisting of 25% ethacrynic acid, no inhibition by Ba2+ | Liposcelis paeta | |
Zn2+ | - |
Liposcelis paeta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | purified enzyme, Michaelis-Menten kinetic analysis, overview | Liposcelis paeta | |
0.68 | - |
glutathione | pH 7.5, 37°C, enzyme of Wuzhou population | Liposcelis paeta | |
0.71 | - |
glutathione | pH 7.5, 37°C, enzymes of Hezhou and Nanyang population | Liposcelis paeta | |
6.4 | - |
1-chloro-2,4-dinitrobenzene | pH 7.5, 37°C, enzyme of Nanyang population | Liposcelis paeta | |
16 | - |
1-chloro-2,4-dinitrobenzene | pH 7.5, 37°C, enzyme of Wuzhou population | Liposcelis paeta | |
223.6 | - |
1-chloro-2,4-dinitrobenzene | pH 7.5, 37°C, enzyme of Hezhou population | Liposcelis paeta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19420 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
20130 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
20430 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
23310 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
50790 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
53140 | - |
1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | Liposcelis paeta | the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Liposcelis paeta | - |
three field populations collected from Nanyang city of Henan Province, and Wuzhou and Hezhou cities of Guangxi Province, China | - |
Purification (Comment) | Organism |
---|---|
native enzyme by glutathione affinity chromatography, three field populations from Nanyang, purification 42.5fold, Wuzhou, purification 64.5fold, and Hezhou purification 67.4fold | Liposcelis paeta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00065 | - |
crude enzyme of Wuzhou population | Liposcelis paeta |
0.0012 | - |
crude enzyme of Nanyang population | Liposcelis paeta |
0.0021 | - |
crude enzyme of Hezhou population | Liposcelis paeta |
0.0494 | - |
purified enzyme of Nanyang population | Liposcelis paeta |
0.0645 | - |
purified enzyme of Wuzhou population | Liposcelis paeta |
0.0674 | - |
purified enzyme of Hezhou population | Liposcelis paeta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene | Liposcelis paeta | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | no activity with 1,2-dichloro-nitrobenzene | Liposcelis paeta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE | Liposcelis paeta |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Liposcelis paeta |
GST | - |
Liposcelis paeta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
enzyme from Nanyang population | Liposcelis paeta |
40 | - |
enzymes from Wuzhou population and from Hezhou population | Liposcelis paeta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 42 | temperature profiles of enzyme from different populations, overview | Liposcelis paeta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
enzyme from Nanyang population | Liposcelis paeta |
7.5 | 8 | enzyme from Wuzhou population | Liposcelis paeta |
8 | - |
enzyme from Hezhou population | Liposcelis paeta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH profiles of enzyme from different populations, overview | Liposcelis paeta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
IC50 values for insecticides and metal ions for the enzymes from different populations, overview | Liposcelis paeta | additional information | |
0.000000395 | 0.000000842 | - |
Liposcelis paeta | Zn2+ | |
0.000000873 | 0.00000585 | - |
Liposcelis paeta | Hg2+ | |
0.00000181 | 0.00000203 | - |
Liposcelis paeta | Ethacrynic acid | |
0.000243 | 0.000583 | - |
Liposcelis paeta | Mg2+ | |
0.00039 | 0.000978 | - |
Liposcelis paeta | cypermethrin | |
0.00132 | 0.00512 | - |
Liposcelis paeta | Mn2+ | |
0.00158 | 0.0284 | - |
Liposcelis paeta | Ca2+ | |
0.0056 | 0.0148 | pH 7.5, 37°C | Liposcelis paeta | chlorpyrifos | |
0.055 | 0.806 | - |
Liposcelis paeta | carbosulfan |