Cloned (Comment) | Organism |
---|---|
Escherichia coli structural gene aroA | Escherichia coli |
General Stability | Organism |
---|---|
dithiothreitol reverses aggregation which sometimes occurs after storage at -20°C | Escherichia coli |
glycerol, 50% v/v, stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | inhibition of enolpyruvate transfer, competitive vs. phosphoenolpyruvate | Escherichia coli | |
N-phosphonomethylglycine | - |
Klebsiella pneumoniae | |
N-phosphonomethylglycine | - |
Neurospora crassa | |
N-phosphonomethylglycine | - |
Pisum sativum | |
N-phosphonomethylglycine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
5-enolpyruvylshikimate 3-phosphate | - |
Neurospora crassa | |
0.00036 | - |
3-phosphoshikimate | - |
Neurospora crassa | |
0.0018 | - |
phosphate | - |
Neurospora crassa | |
0.0025 | - |
3-phosphoshikimate | recombinant overproducing strain | Escherichia coli | |
0.0027 | - |
phosphoenolpyruvate | - |
Neurospora crassa | |
0.003 | - |
5-enolpyruvylshikimate 3-phosphate | recombinant overproducing strain | Escherichia coli | |
0.0052 | - |
phosphoenolpyruvate | cosubstrate 5-enolpyruvylshikimate 3-phosphate | Pisum sativum | |
0.0077 | - |
3-phosphoshikimate | - |
Pisum sativum | |
0.016 | - |
phosphoenolpyruvate | recombinant overproducing strain | Escherichia coli | |
2.5 | - |
phosphate | - |
Escherichia coli | |
4 | - |
phosphate | - |
Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
soluble | - |
Escherichia coli | - |
- |
soluble | - |
Neurospora crassa | - |
- |
soluble | - |
Klebsiella pneumoniae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Escherichia coli |
46110 | - |
calculated from polypeptide sequence of aroA gene product | Escherichia coli |
49000 | - |
1 * 49000 | Escherichia coli |
55000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Neurospora crassa | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Pisum sativum | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | sixth reaction of chorismate pathway | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
overproducing recombinant strain AB2829pKD501 | - |
Klebsiella pneumoniae | - |
- |
- |
Neurospora crassa | - |
- |
- |
Pisum sativum | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, phosphocellulose chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate, native enzyme | Escherichia coli |
62 | - |
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, in 50% v/v glycerol, enzyme concentration 5 mg/ml, at least 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Neurospora crassa | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Pisum sativum | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Neurospora crassa | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Pisum sativum | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Klebsiella pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | sixth reaction of chorismate pathway | Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pisum sativum |
monomer | - |
Klebsiella pneumoniae |
monomer | 1 * 49000 | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Neurospora crassa |
25 | - |
assay at | Pisum sativum |