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Literature summary for 2.5.1.19 extracted from

  • Lewendon, A.; Coggins, J.R.
    3-Phosphoshikimate 1-carboxyvinyltransferase from Escherichia coli (1987), Methods Enzymol., 142, 342-348.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli structural gene aroA Escherichia coli

General Stability

General Stability Organism
dithiothreitol reverses aggregation which sometimes occurs after storage at -20°C Escherichia coli
glycerol, 50% v/v, stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
N-phosphonomethylglycine inhibition of enolpyruvate transfer, competitive vs. phosphoenolpyruvate Escherichia coli
N-phosphonomethylglycine
-
Klebsiella pneumoniae
N-phosphonomethylglycine
-
Neurospora crassa
N-phosphonomethylglycine
-
Pisum sativum
N-phosphonomethylglycine
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
5-enolpyruvylshikimate 3-phosphate
-
Neurospora crassa
0.00036
-
3-phosphoshikimate
-
Neurospora crassa
0.0018
-
phosphate
-
Neurospora crassa
0.0025
-
3-phosphoshikimate recombinant overproducing strain Escherichia coli
0.0027
-
phosphoenolpyruvate
-
Neurospora crassa
0.003
-
5-enolpyruvylshikimate 3-phosphate recombinant overproducing strain Escherichia coli
0.0052
-
phosphoenolpyruvate cosubstrate 5-enolpyruvylshikimate 3-phosphate Pisum sativum
0.0077
-
3-phosphoshikimate
-
Pisum sativum
0.016
-
phosphoenolpyruvate recombinant overproducing strain Escherichia coli
2.5
-
phosphate
-
Escherichia coli
4
-
phosphate
-
Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
soluble
-
Escherichia coli
-
-
soluble
-
Neurospora crassa
-
-
soluble
-
Klebsiella pneumoniae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Escherichia coli
46110
-
calculated from polypeptide sequence of aroA gene product Escherichia coli
49000
-
1 * 49000 Escherichia coli
55000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Salmonella enterica subsp. enterica serovar Typhimurium
-
phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate Neurospora crassa
-
phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate Pisum sativum
-
phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli sixth reaction of chorismate pathway phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-
Escherichia coli
-
overproducing recombinant strain AB2829pKD501
-
Klebsiella pneumoniae
-
-
-
Neurospora crassa
-
-
-
Pisum sativum
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, phosphocellulose chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
phosphate + 5-enolpyruvylshikimate 3-phosphate, native enzyme Escherichia coli
62
-
phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, in 50% v/v glycerol, enzyme concentration 5 mg/ml, at least 1 year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate
-
Neurospora crassa phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate
-
Pisum sativum phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?
phosphoenolpyruvate + 3-phosphoshikimate
-
Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate
-
Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate
-
Neurospora crassa phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate
-
Pisum sativum phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate
-
Klebsiella pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate sixth reaction of chorismate pathway Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer
-
Pisum sativum
monomer
-
Klebsiella pneumoniae
monomer 1 * 49000 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli
25
-
assay at Neurospora crassa
25
-
assay at Pisum sativum