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Literature summary for 2.5.1.19 extracted from
- 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties (1984), Eur. J. Biochem., 143, 341-349.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Br- | stimulation at low concentrations, inhibition above 200 mM | Klebsiella pneumoniae |  |
| Cl- | activation up to 100 mM, inhibition above | Klebsiella pneumoniae | |
| F- | stimulation | Klebsiella pneumoniae | |
| I- | stimulation at low concentrations, inhibition above 100 mM | Klebsiella pneumoniae | |
| NH4Cl | stimulation, inhibition above 300 mM | Klebsiella pneumoniae | |
| NO3- | stimulation at low concentrations, inhibition above 15 mM | Klebsiella pneumoniae | |
General Stability
| General Stability | Organism |
|---|---|
| EDTA stabilizes | Klebsiella pneumoniae |
| freezing inactivates, Polybuffer 74, operating range pH 7-4, 1 mM dithioerythritol stabilize | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-enolpyruvylshikimate 3-phosphate | product inhibition | Klebsiella pneumoniae | |
| Al3+ | 10 mM, 37% inhibition | Klebsiella pneumoniae | |
| Br- | inhibition above 200 mM, stimulation below | Klebsiella pneumoniae | |
| Cl- | inhibition above 400 mM, stimulation below | Klebsiella pneumoniae | |
| Co3+ | 10 mM, 25% inhibition | Klebsiella pneumoniae | |
| Cu2+ | 10 mM, 90-95% inhibition | Klebsiella pneumoniae | |
| Fe2+ | 10 mM, complete inhibition | Klebsiella pneumoniae | |
| Fe3+ | 10 mM, 60% inhibition | Klebsiella pneumoniae | |
| I- | inhibition above 100 mM, stimulation below | Klebsiella pneumoniae | |
| La3+ | 10 mM, complete inhibition | Klebsiella pneumoniae | |
| Mn2+ | 10 mM, 16% inhibition | Klebsiella pneumoniae | |
| molybdate | 10 mM, complete inhibition | Klebsiella pneumoniae | |
| NH4Cl | inhibition above 300 mM, stimulation below | Klebsiella pneumoniae | |
| NO3- | inhibition above 150 mM, stimulation below | Klebsiella pneumoniae | |
| Pb2+ | 10 mM, 90-95% inhibition | Klebsiella pneumoniae | |
| phosphoenolpyruvate | - |
Klebsiella pneumoniae | |
| shikimate 3-phosphate | product inhibition | Klebsiella pneumoniae | |
| Zn2+ | 10 mM, 90-95% inhibition | Klebsiella pneumoniae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Klebsiella pneumoniae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Klebsiella pneumoniae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by Ba2+, Ca2+, Mg2+, Ni2+ or Sr2+ | Klebsiella pneumoniae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32400 | - |
gel filtration | Klebsiella pneumoniae |
| 42900 | - |
1 * 42900, glyphosate-resistant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.8 | - |
- |
Klebsiella pneumoniae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, in Polybuffer 74 from Pharmacia, operating range pH 7-4, 1 mM dithioerythritol, at least 9 months | Klebsiella pneumoniae |
| 0-4°C, 2 months | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Klebsiella pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42900, glyphosate-resistant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Klebsiella pneumoniae |
| 60 | - |
wild-type enzyme | Klebsiella pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 62 | approx. half-maximal activity at 45°C and 62°C | Klebsiella pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
rapid inactivation above | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.6, wild-type enzyme | Klebsiella pneumoniae |
| 5.6 | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate, 2 maxima at pH 5.6 and pH 7.6 | Klebsiella pneumoniae |
| 7.6 | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate, 2 maxima, at pH 5.6 and pH 7.6 | Klebsiella pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | approx. half-maximal activity at pH 5.0 and 8.5 | Klebsiella pneumoniae |
| 5.5 | 8.7 | approx. half-maximal activity at pH 5.5 and 8.7, phosphate + 5-enolpyruvylshikimate 3-phosphate | Klebsiella pneumoniae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
5-enolpyruvylshikimate 3-phosphate | - |
Klebsiella pneumoniae | |
| 0.023 | - |
shikimate 3-phosphate | - |
Klebsiella pneumoniae | |
| 0.044 | - |
phosphoenolpyruvate | - |
Klebsiella pneumoniae | |
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