Cloned (Comment) | Organism |
---|---|
expression of wild-type and chimeras of Salmonella and Escherichia coli mutant enzymes in Escherichia coli | Escherichia coli |
expression of wild-type and chimeras of Salmonella typhimurium and Escherichia coli mutant enzymes in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | - |
Escherichia coli | |
N-phosphonomethylglycine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.5 | - |
phosphoenolpyruvate | recombinant enzyme, in crude extracts | Escherichia coli | |
43.6 | - |
phosphoenolpyruvate | recombinant enzyme, in crude extracts | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0058 | - |
recombinant enzyme, activity in crude extracts | Salmonella enterica subsp. enterica serovar Typhimurium |
0.0064 | - |
recombinant enzyme, activity in crude extracts | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
N-phosphonomethylglycine | recombinant enzyme, in crude extracts | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.96 | - |
N-phosphonomethylglycine | recombinant enzyme, in crude extracts | Escherichia coli |