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Literature summary for 2.5.1.19 extracted from

  • He, M.; Yang, Z.Y.; Nie, Y.F.; Wang, J.; Xu, P.
    A new type of class I bacterial 5-enopyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate (2001), Biochim. Biophys. Acta, 1568, 1-6.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and chimeras of Salmonella and Escherichia coli mutant enzymes in Escherichia coli Escherichia coli
expression of wild-type and chimeras of Salmonella typhimurium and Escherichia coli mutant enzymes in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
N-phosphonomethylglycine
-
Escherichia coli
N-phosphonomethylglycine
-
Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22.5
-
phosphoenolpyruvate recombinant enzyme, in crude extracts Escherichia coli
43.6
-
phosphoenolpyruvate recombinant enzyme, in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0058
-
recombinant enzyme, activity in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium
0.0064
-
recombinant enzyme, activity in crude extracts Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-enolpyruvylshikimate 3-phosphate
-
r
phosphoenolpyruvate + 3-phosphoshikimate
-
Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.54
-
N-phosphonomethylglycine recombinant enzyme, in crude extracts Salmonella enterica subsp. enterica serovar Typhimurium
0.96
-
N-phosphonomethylglycine recombinant enzyme, in crude extracts Escherichia coli