Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-isobutylthioadenosine | - |
Saccharolobus solfataricus | |
5'-methylthiotubercidin | - |
Saccharolobus solfataricus | |
5'-S-ethyl-5'-thioadenosine | - |
Saccharolobus solfataricus | |
5'-S-methyl-5'-thioadenosine | powerful competitive inhibitor | Saccharolobus solfataricus | |
A9154C | - |
Saccharolobus solfataricus | |
n-propylthioadenosine | - |
Saccharolobus solfataricus | |
S-adenosyl(5')-3-thiopropylamine | - |
Saccharolobus solfataricus | |
S-adenosyl-3-thio-1,8-diaminooctane | - |
Saccharolobus solfataricus | |
S-adenosyl-3-thiopropylamine | - |
Saccharolobus solfataricus | |
sinefungin | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
S-adenosylmethioninamine | pH 7.5, 78°C | Saccharolobus solfataricus | |
0.954 | - |
sym-norspermidine | pH 7.5, 78°C | Saccharolobus solfataricus | |
1.539 | - |
spermidine | pH 7.5, 78°C | Saccharolobus solfataricus | |
1.675 | - |
propane-1,3-diamine | pH 7.5, 78°C | Saccharolobus solfataricus | |
3.85 | - |
putrescine | pH 7.5, 78°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | no activity is associated with the cellular particulate fraction | Saccharolobus solfataricus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
3 * 35000, SDS-PAGE | Saccharolobus solfataricus |
110000 | - |
gel filtration, ultracentrifugation analysis | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT-4 / DSM 5833 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.059 | - |
pH 7.5, 78°C | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, pH 7.5, stable for 1 months | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + propane-1,3-diamine | - |
Saccharolobus solfataricus | 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
ir | |
S-adenosylmethioninamine + propane-1,3-diamine | - |
Saccharolobus solfataricus MT-4 / DSM 5833 | 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
ir | |
S-adenosylmethioninamine + putrescine | 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus | 5'-S-methyl-5'-thioadenosine + spermidine | - |
ir | |
S-adenosylmethioninamine + putrescine | 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus MT-4 / DSM 5833 | 5'-S-methyl-5'-thioadenosine + spermidine | - |
ir | |
S-adenosylmethioninamine + spermidine | 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus | 5'-S-methyl-5'-thioadenosine + spermine | - |
ir | |
S-adenosylmethioninamine + spermidine | 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus MT-4 / DSM 5833 | 5'-S-methyl-5'-thioadenosine + spermine | - |
ir | |
S-adenosylmethioninamine + sym-norspermidine | 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus | 5'-S-methyl-5'-thioadenosine + ? | - |
ir | |
S-adenosylmethioninamine + sym-norspermidine | 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism | Saccharolobus solfataricus MT-4 / DSM 5833 | 5'-S-methyl-5'-thioadenosine + ? | - |
ir |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 35000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
assay at | Saccharolobus solfataricus |
90 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 110 | 50°C: about 40% of maximal activity, 110°C: about 45% of maximal activity, 120°C: about 20% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
1 h, completely stable | Saccharolobus solfataricus |
120 | - |
no protection by substrate against thermal inactivation at 120°C is observed by incubating the enzyme in the presence of polyamines | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6: about 50% of maximal activity, pH 9.5: about 50% of maximal activity | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
5'-S-methyl-5'-thioadenosine | pH 7.5, 78°C | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
5.3 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.014 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | 5'-S-methyl-5'-thioadenosine | |
0.016 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | S-adenosyl-3-thiopropylamine | |
0.021 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | S-adenosyl-3-thio-1,8-diaminooctane | |
0.1 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | sinefungin | |
0.1 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | 5'-S-ethyl-5'-thioadenosine | |
0.1 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | A9154C | |
0.12 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | n-propylthioadenosine | |
0.15 | - |
pH 7.5, 78°C | Saccharolobus solfataricus | 5'-isobutylthioadenosine |
Organism | Comment | Expression |
---|---|---|
Saccharolobus solfataricus | no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium | additional information |