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Literature summary for 2.5.1.27 extracted from

  • Chen, C.; Melitz, D.K.
    Cytokinin biosynthesis in a cell-free system from cytokinin-autotrophic tobacco tissue cultures (1979), FEBS Lett., 107, 15-20.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + AMP Nicotiana tabacum biosynthesis of cytokinins diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
callus cytokinin-autotrophic Nicotiana tabacum
-

Storage Stability

Storage Stability Organism
-70°C, about 90% loss of activity after 3 weeks Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + AMP
-
Nicotiana tabacum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
?
dimethylallyl diphosphate + AMP biosynthesis of cytokinins Nicotiana tabacum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Nicotiana tabacum