Literature summary for 2.5.1.29 extracted from

Noike, M.; Katagiri, T.; Nakayama, T.; Koyama, T.; Nishino, T.; Hemmi, H.
The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction (2008), FEBS J., 275, 3921-3933.

Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment)	Organism
GGPS and the mutant enzymes are expressed in Escherichia coli as inclusion bodies	Pantoea ananatis

Protein Variants

Protein Variants	Comment	Organism
A89F	mutant enzyme yields shorter products than the wild-type enzyme. Substrate specificity is almost identical to that of farnesyl diphosphate synthase	Pantoea ananatis
A89H	mutant enzyme yields shorter products than the wild-type enzyme. Substrate specificity is almost identical to that of farnesyl diphosphate synthase	Pantoea ananatis
A89L	mutant enzyme yields shorter products than the wild-type enzyme	Pantoea ananatis
H87A	activity using geranylgeranyl diphosphate as the substrate is undetectable	Pantoea ananatis
I121A	mutant enzyme yields longer products than the wild-type enzyme	Pantoea ananatis
L127A	approximately 20% of wild-type activity	Pantoea ananatis
L128A	mutant enzyme is completely inactive	Pantoea ananatis
V125A	mutant enzyme yields longer products than the wild-type enzyme	Pantoea ananatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130000	-	tetramer, non-denaturing PAGE	Pantoea ananatis
240000	-	octamer, non-denaturing PAGE	Pantoea ananatis

Organism

Organism	UniProt	Comment	Textmining
Pantoea ananatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pantoea ananatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
farnesyl diphosphate + isopentenyl diphosphate	type II geranylgeranyl diphosphate synthase utilizes a mechanism of chain length determination, which requires subunit interaction in the homooligomeric enzyme	Pantoea ananatis	diphosphate + geranylgeranyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
GGPS	-	Pantoea ananatis
type II geranylgeranyl diphosphate synthase	-	Pantoea ananatis

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Release 2023.1 (January 2023)