Literature summary for 2.5.1.29 extracted from

Zhou, P.; Xie, W.; Li, A.; Wang, F.; Yao, Z.; Bian, Q.; Zhu, Y.; Yu, H.; Ye, L.
Alleviation of metabolic bottleneck by combinatorial engineering enhanced astaxanthin synthesis in Saccharomyces cerevisiae (2017), Enzyme Microb. Technol., 100, 28-36 .

Cloned(Commentary)

Cloned (Comment)	Organism
astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution	Phaffia rhodozyma

Cloned (Comment)

Organism

astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution

Phaffia rhodozyma

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate	Phaffia rhodozyma	the enzyme is involved in the astaxanthin biosynthesis pathway	diphosphate + geranylgeranyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate	Phaffia rhodozyma CGMCC As2.1557	the enzyme is involved in the astaxanthin biosynthesis pathway	diphosphate + geranylgeranyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Phaffia rhodozyma	Q1L6K3	-	-
Phaffia rhodozyma CGMCC As2.1557	Q1L6K3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate	the enzyme is involved in the astaxanthin biosynthesis pathway	Phaffia rhodozyma	diphosphate + geranylgeranyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate	the enzyme is involved in the astaxanthin biosynthesis pathway	Phaffia rhodozyma CGMCC As2.1557	diphosphate + geranylgeranyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
BTS1	-	Phaffia rhodozyma
CrtE	-	Phaffia rhodozyma
GGPP synthase	-	Phaffia rhodozyma

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the astaxanthin biosynthesis pathway	Phaffia rhodozyma