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Literature summary for 2.5.1.3 extracted from

  • Kawasaki, Y.
    Copurification of hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase of Saccharomyces cerevisiae: characterization of hydroxyethylthiazole kinase as a bifunctional enzyme in the thiamine biosynthetic pathway (1993), J. Bacteriol., 175, 5153-5158.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.3

Inhibitors

Inhibitors Comment Organism Structure
PCMB 0.01 mM, 98.5% inhibition, inhibition prevented by addition of 2-mercaptoethanol in 10fold molar excess Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 8 * 60000, SDS-PAGE, hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae
470000
-
 hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate Saccharomyces cerevisiae the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole diphosphate + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 mutant resistant to 2-amino-4-methyl-5-beta-hydroxyethylthiazole, an antimetabolite of 4-methyl-5-beta-hydroxyethylthiazole, deficient in activity of both EC 2.5.1.3 and EC 2.7.1.50
-
Saccharomyces cerevisiae
-
 IFO 10483
-
Saccharomyces cerevisiae
-
 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-

Purification (Commentary)

Purification (Comment) Organism
bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.269
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, about 64.4% loss of activity after 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate the bifunctional enzyme hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase catalyzes two sequential steps in the synthesis of thiamin monophosphate from hydroxyethylthiazole Saccharomyces cerevisiae diphosphate + ?
-
 ?
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
-
 Saccharomyces cerevisiae thiamine monophosphate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 60000, SDS-PAGE, hydroxyethylthiazole kinase is a bifunctional enzyme with thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 9.1 pH 7.0: about 30% of maximal activity, pH 9.1: optimum Saccharomyces cerevisiae