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Literature summary for 2.5.1.31 extracted from
- Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase (1982), J. Biochem., 92, 1527-1537.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| polyamines | stimulate in presence of Mg2+ | Bacillus subtilis | |
| putrescine | stimulates | Bacillus subtilis |  |
| Triton X-100 | - |
Bacillus subtilis | |
| Tween 80 | stimulates | Bacillus subtilis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | 20 mM, 20% inhibition | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0091 | - |
(2E,6E)-farnesyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0091 | - |
(E,E)-farnesyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0093 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0093 | - |
(E,E,E)-geranylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0154 | - |
(2Z,6E,10E)-geranylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0154 | - |
(Z,E,E)-Geranylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0189 | - |
(Z,E)-farnesyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0267 | - |
neryl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0526 | - |
(Z,Z,E,E)-farnesylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0526 | - |
(2Z,6Z,10E,14E)-farnesylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0588 | - |
(Z,E,E,E)-farnesylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
| 0.0588 | - |
(2Z,6E,10E,14E)-farnesylgeranyl diphosphate | pH 8.5, 37°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
| Mg2+ | divalent cation required | Bacillus subtilis | |
| Mg2+ | optimal activity at 5 mM, required | Bacillus subtilis | |
| Mn2+ | can partially replace Mg2+ | Bacillus subtilis | |
| Mn2+ | can partially replace Mg2+, optimal activity at 0.5 mM | Bacillus subtilis | |
| Na+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
| NH4+ | stimulates in presence of Mg2+ | Bacillus subtilis | |
| Zn2+ | can partially replace Mg2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Bacillus subtilis |
| 85000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
| partially purified | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000424 | - |
- |
Bacillus subtilis |
| 0.000424 | - |
pH 8.5, 37°C | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 24 h, 56% loss of activity, partially purified enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Bacillus subtilis | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
| (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (2Z,6E)-famesyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (2Z,6E,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (2Z,6Z,10E,14E)-farnesylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (Z,E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| (Z,E,E,E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
| isopentenyl diphosphate + farnesyl diphosphate | (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate | ? | |
| isopentenyl diphosphate + farnesyl diphosphate | (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry | ? | |
| isopentenyl diphosphate + farnesyl diphosphate | (E,E)-farnesyl diphosphate or (Z,E)-farnesyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate | ? | |
| isopentenyl diphosphate + farnesyl diphosphate | (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 3:1 from (Z,E,E,E)-farnesylgeranyl diphosphate and in the ratio 3:2 from (Z,Z,E,E)-farnesylgeranyl diphosphate | ? | |
| isopentenyl diphosphate + farnesyl diphosphate | (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C50-polyprenyl diphosphate and C55-polyprenyl diphosphate with Z,E mixed stereochemistry | ? | |
| isopentenyl diphosphate + farnesyl diphosphate | (Z,E,E,E)-farnesylgeranyl diphosphate or (Z,Z,E,E)-farnesylgeranyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | formation of C50-polyprenyl diphosphate and C55-polyprenyl diphosphate in the ratio of 1:4 from (E,E)-farnesyl diphosphate and in the ratio of 4:1 from (Z,E)-farnesyl diphosphate | ? | |
| isopentenyl diphosphate + geranyl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | - |
? | |
| isopentenyl diphosphate + geranylgeranyl diphosphate | (E,E,E)-geranylgeranyl diphosphate or (Z,E,E)-geranylgeranyl diphosphate | Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C50-prenyl diphosphate and C55-prenyl diphosphate in the ratio of 2:3 from (E,E,E)-geranyl diphosphate and in the ratio 1:3 from (Z,E,E)-geranylgeranyl diphosphate | ? | |
| isopentenyl diphosphate + neryl diphosphate | - |
Bacillus subtilis | C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate | C45 prenyl diphosphate, C50 prenyl diphosphate and C55 prenyl diphosphate in the ratio of 2:10:1 | ? | |
| neryl diphosphate + isopentenyl diphosphate | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| undecaprenyl pyrophosphate synthetase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus subtilis |
| 8.5 | - |
assay at | Bacillus subtilis |
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