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Literature summary for 2.5.1.31 extracted from

  • Baba, T.; Allen, C.M.
    Prenyl transferases from Micrococcus luteus: characterization of undecaprenyl pyrophosphate synthetase (1980), Arch. Biochem. Biophys., 200, 474-484.
    View publication on PubMed
Search for more literature for 2.5.1.31

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin poor activator Micrococcus luteus
deoxycholate poor activator Micrococcus luteus
lecithin from egg, stimulates Micrococcus luteus
lecithin 14fold stimulation at 2 mM Micrococcus luteus
additional information enzyme is inactive in absence of added effectors Micrococcus luteus
Phospholipid phospholipid extract from Micrococcus luteus stimulates Micrococcus luteus
Triton X-100 stimulates Micrococcus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 (E,E)-farnesyl diphosphate pH 7.4, 35°C Micrococcus luteus
0.008
-
 (E,E,E)-geranylgeranyl diphosphate pH 7.5, 35°C Micrococcus luteus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Micrococcus luteus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus
Mg2+ maximal stimulation at 0.1 mM Micrococcus luteus
Mn2+ enzyme is optimally stimulated by 0.1 mM Mg2+, 0.05 mM Mn2+ or 0.2 mM Co2+ Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000 49000 gel filtration Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004066
-
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
isopentenyl diphosphate + DELTA3-isopentenyl diphosphate
-
 Micrococcus luteus ?
-
 ?
isopentenyl diphosphate + farnesyl diphosphate cis,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate trans,trans-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + geranylgeranyl diphosphate trans,trans,trans-geranylgeranyl diphosphate and cis,trans,trans-geranylgeranyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
isopentenyl diphosphate + neryl diphosphate
-
 Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?
additional information reactivities of the allylic substrates increase with chain length: C10, C15, C20. Each new isoprene unit added has a cis-configuration Micrococcus luteus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Micrococcus luteus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: activity maximum, pH 9.0: about 80% of maximal activity Micrococcus luteus