KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
prephytoene diphosphate | pH 7.6, 30°C | Capsicum annuum | |
0.003 | - |
geranylgeranyl diphosphate | pH 7.6, 30°C | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromoplast | stroma | Capsicum annuum | 9509 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | absolute requirement for Mn2+, half-maximal activity at 0.2 mM. No other divalent cation can substitute efficiently for Mn2+ | Capsicum annuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
1 * 47500, SDS-PAGE | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | - |
a single polypeptide catalyzes the 2-step reaction from geranylgeranyl diphosphate to phytoene | - |
Purification (Comment) | Organism |
---|---|
- |
Capsicum annuum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Capsicum annuum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.067 | - |
- |
Capsicum annuum |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl buffer, pH 7.6, 2 mM dithiothreitol, 0.3 M NaCl, 30% glycerol, stable for 1 year | Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + geranylgeranyl diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Capsicum annuum | prephytoene diphosphate + diphosphate | - |
? | |
prephytoene diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Capsicum annuum | phytoene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47500, SDS-PAGE | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Capsicum annuum |