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Literature summary for 2.5.1.32 extracted from

  • Fraser, P.D.; Schuch, W.; Bramley, P.M.
    Phytoene synthase from tomato (Lycopersicon esculentum) chloroplasts - partial purification and biochemical properties (2000), Planta, 211, 361-369.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP Mn2+ and ATP are essential for catalytic activity, the effect is stoichiometric from 0.5-2 mM. Km for ATP is 2.0 mM Solanum lycopersicum
Triton X-100 0.1 w/v, 5fold stimulation Solanum lycopersicum
Tween 60 0.1 w/v, 5fold stimulation Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Solanum lycopersicum
AMP
-
Solanum lycopersicum
beta-carotene
-
Solanum lycopersicum
Chlorophyll
-
Solanum lycopersicum
GTP
-
Solanum lycopersicum
Mn2+ above 2 mM Solanum lycopersicum
NADP+
-
Solanum lycopersicum
NADPH
-
Solanum lycopersicum
phosphate
-
Solanum lycopersicum
squalestatin
-
Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
geranylgeranyl diphosphate pH 8.0, 28°C, enzyme from chloroplast Solanum lycopersicum
0.01
-
geranylgeranyl diphosphate pH 8.0, 28°C, enzyme from chromoplast Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated. Treatment with high ionic strength buffer or mild non-ionic detergent is required for solubilization Solanum lycopersicum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+ and ATP in combination are essential for catalytic activity, the effect is stoichiometric from 0.5-2.0 mM. Km for Mn2+ is 0.4 mM Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
native PAGE Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Solanum lycopersicum
-
leaf low activity in young leaf Solanum lycopersicum
-

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris, pH 7.5, 10% v/v glycerol, 80% of the activity retained Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate
-
Solanum lycopersicum prephytoene diphosphate + diphosphate
-
?

Subunits

Subunits Comment Organism
More the enzyme is functional in a monomeric state, under optimal native conditions it is associated with a large protein complex, at least 200000 Da, which contains other terpenoid enzymes Solanum lycopersicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
10 h, stable Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
enzyme from chloroplast Solanum lycopersicum
7.5
-
enzyme from chromoplast Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000007
-
squalestatin pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
0.000009
-
squalestatin pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
0.0002
-
NADP+ pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme Solanum lycopersicum
0.1
-
NADPH pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
0.2
-
phosphate pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme Solanum lycopersicum
0.4
-
NADPH pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
0.5
-
phosphate pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
1
-
AMP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
3
-
ADP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
4.3
-
GTP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
4.3
-
ADP pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum