Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | Mn2+ and ATP are essential for catalytic activity, the effect is stoichiometric from 0.5-2 mM. Km for ATP is 2.0 mM | Solanum lycopersicum | |
Triton X-100 | 0.1 w/v, 5fold stimulation | Solanum lycopersicum | |
Tween 60 | 0.1 w/v, 5fold stimulation | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Solanum lycopersicum | |
AMP | - |
Solanum lycopersicum | |
beta-carotene | - |
Solanum lycopersicum | |
Chlorophyll | - |
Solanum lycopersicum | |
GTP | - |
Solanum lycopersicum | |
Mn2+ | above 2 mM | Solanum lycopersicum | |
NADP+ | - |
Solanum lycopersicum | |
NADPH | - |
Solanum lycopersicum | |
phosphate | - |
Solanum lycopersicum | |
squalestatin | - |
Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
geranylgeranyl diphosphate | pH 8.0, 28°C, enzyme from chloroplast | Solanum lycopersicum | |
0.01 | - |
geranylgeranyl diphosphate | pH 8.0, 28°C, enzyme from chromoplast | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-associated. Treatment with high ionic strength buffer or mild non-ionic detergent is required for solubilization | Solanum lycopersicum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+ and ATP in combination are essential for catalytic activity, the effect is stoichiometric from 0.5-2.0 mM. Km for Mn2+ is 0.4 mM | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
native PAGE | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
leaf | low activity in young leaf | Solanum lycopersicum | - |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris, pH 7.5, 10% v/v glycerol, 80% of the activity retained | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + geranylgeranyl diphosphate | - |
Solanum lycopersicum | prephytoene diphosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is functional in a monomeric state, under optimal native conditions it is associated with a large protein complex, at least 200000 Da, which contains other terpenoid enzymes | Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
10 h, stable | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
enzyme from chloroplast | Solanum lycopersicum |
7.5 | - |
enzyme from chromoplast | Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000007 | - |
squalestatin | pH 8.0, 28°C, chloroplast enzyme | Solanum lycopersicum | |
0.000009 | - |
squalestatin | pH 8.0, 28°C, chromoplast enzyme | Solanum lycopersicum | |
0.0002 | - |
NADP+ | pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme | Solanum lycopersicum | |
0.1 | - |
NADPH | pH 8.0, 28°C, chloroplast enzyme | Solanum lycopersicum | |
0.2 | - |
phosphate | pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme | Solanum lycopersicum | |
0.4 | - |
NADPH | pH 8.0, 28°C, chromoplast enzyme | Solanum lycopersicum | |
0.5 | - |
phosphate | pH 8.0, 28°C, chromoplast enzyme | Solanum lycopersicum | |
1 | - |
AMP | pH 8.0, 28°C, chloroplast enzyme | Solanum lycopersicum | |
3 | - |
ADP | pH 8.0, 28°C, chloroplast enzyme | Solanum lycopersicum | |
4.3 | - |
GTP | pH 8.0, 28°C, chloroplast enzyme | Solanum lycopersicum | |
4.3 | - |
ADP | pH 8.0, 28°C, chromoplast enzyme | Solanum lycopersicum |