Cloned (Comment) | Organism |
---|---|
astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution | Phaffia rhodozyma |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 geranylgeranyl diphosphate | Phaffia rhodozyma | the enzyme is involved in the astaxanthin biosynthesis pathway | 15-cis-phytoene + 2 diphosphate | - |
? | |
2 geranylgeranyl diphosphate | Phaffia rhodozyma CGMCC As2.1557 | the enzyme is involved in the astaxanthin biosynthesis pathway | 15-cis-phytoene + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaffia rhodozyma | Q7Z859 | - |
- |
Phaffia rhodozyma CGMCC As2.1557 | Q7Z859 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 geranylgeranyl diphosphate | the enzyme is involved in the astaxanthin biosynthesis pathway | Phaffia rhodozyma | 15-cis-phytoene + 2 diphosphate | - |
? | |
2 geranylgeranyl diphosphate | the enzyme is involved in the astaxanthin biosynthesis pathway | Phaffia rhodozyma CGMCC As2.1557 | 15-cis-phytoene + 2 diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
crtYB | - |
Phaffia rhodozyma |
phytoene synthase/lycopene cyclase | - |
Phaffia rhodozyma |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the astaxanthin biosynthesis pathway | Phaffia rhodozyma |