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Literature summary for 2.5.1.39 extracted from

  • Gupta, A.; Rudney, H.
    4-Hydroxybenzoate polyprenyltransferase from rat liver (1985), Methods Enzymol., 110, 327-334.
    View publication on PubMed
Search for more literature for 2.5.1.39

Activating Compound

Activating Compound Comment Organism Structure
Brij W1 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
Nonidet P-40 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
sodium deoxycholate inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
Triton X-100 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus
Tween 80 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus

General Stability

General Stability Organism
isolated mitochondria can be stored frozen for 2-3 weeks prior to preparation of membrane fragments without any loss of activity, preparation of mitochondrial fragments loses activity after 2 cycles of freezing and thawing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-Aminobenzoic acid
-
 Rattus norvegicus
4-Chlorobenzoic acid
-
 Rattus norvegicus
bacitracin strong inhibitor Rattus norvegicus
Brij W1 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
Ca2+ Mg2+ can overcome the inhibition Rattus norvegicus
Diphenylamine
-
 Rattus norvegicus
iodoacetamide
-
 Rattus norvegicus
KCN
-
 Rattus norvegicus
NaF
-
 Rattus norvegicus
Nonidet P-40 inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
p-chloromercuribenzoate
-
 Rattus norvegicus
sodium deoxycholate inhibition at 0.5% or more, activation at 0.05% Rattus norvegicus
Triton X-100 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus
Tween 80 inhibition at 0.5% or more, activation at 0.01% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000833
-
 nonaprenyl diphosphate pH 7.4, 25°C or 37°C Rattus norvegicus
0.00114
-
 4-hydroxybenzoate pH 7.4, 25°C or 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Rattus norvegicus 5743
-
mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Rattus norvegicus
Mg2+ maximal activation: 10 mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
solanesyl diphosphate + 4-hydroxybenzoate Rattus norvegicus key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate diphosphate + 3-solanesyl-4-hydroxybenzoate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Sprague-Dawley
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
solanesyl diphosphate + 4-hydroxybenzoate i.e. nonaprenyl diphosphate Rattus norvegicus diphosphate + 3-solanesyl-4-hydroxybenzoate small amounts of decaprenyl-4-hydroxybenzoate and octaprenylhydroxybenzoate can be found, depending on source of enzyme and substrate used to provide polyprenyl side chains ?
solanesyl diphosphate + 4-hydroxybenzoate key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate Rattus norvegicus diphosphate + 3-solanesyl-4-hydroxybenzoate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Rattus norvegicus