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Literature summary for 2.5.1.44 extracted from
- Biosynthesis of pyrrolizidine alkaloids: putrescine and spermidine are essential substrates of enzymatic homospermidine formation (1994), Can. J. Chem., 72, 80-85.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
putrescine | - |
Blastochloris viridis |  |
| 1.5 | - |
spermidine | in presence of 1 mM putrescine | Blastochloris viridis | |
| 1.7 | - |
putrescine | in presence of 1 mM spermidine | Blastochloris viridis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | Blastochloris viridis | - |
sym-homospermidine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastochloris viridis | - |
DSM 134 | - |
| Blastochloris viridis | - |
accession no. L77975 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Blastochloris viridis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Blastochloris viridis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | 1.2 | - |
Blastochloris viridis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, storage as acetone dry-powder, without significant loss of activity | Blastochloris viridis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 putrescine | - |
Blastochloris viridis | sym-homospermidine + NH3 | - |
? | |
| putrescine + 1,3-diaminopropane | - |
Blastochloris viridis | homospermidine + spermidine | low but significant amounts of homologous polyamines in addition to homospermidine | ? | |
| putrescine + 1,6 diaminohexane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,6-diaminohexane | low but significant amounts of homologous polyamines in addition to homospermidine | ? | |
| putrescine + 1,7-diaminoheptane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane | low but significant amounts of homologous polyamines in addition to homospermidine | ? | |
| putrescine + cadaverine | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,5-diaminopentane | low but significant amounts of homologous polyamines in addition to homospermidine | ? | |
| spermidine | - |
Blastochloris viridis | homospermidine + putrescine + 1,3-diaminopropane | - |
? | |
| spermidine + 1,6-diaminohexane | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,6-diaminohexane | - |
? | |
| spermidine + cadaverine | - |
Blastochloris viridis | homospermidine + N-(4-aminobutyl)-1,5-diaminopentane | - |
? | |
| spermidine + putrescine | - |
Blastochloris viridis | homospermidine + 1,3-diaminopropane | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
stable for at least several weeks | Blastochloris viridis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NAD+ seems to function as hydride acceptor in the first part of the reaction and subsequently as hydride donor in the second part | Blastochloris viridis | |
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