Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.44 extracted from

  • Shaw, F.L.; Elliott, K.A.; Kinch, L.N.; Fuell, C.; Phillips, M.A.; Michael, A.J.
    Evolution and multifarious horizontal transfer of an alternative biosynthetic pathway for the alternative polyamine sym-homospermidine (2010), J. Biol. Chem., 285, 14711-14723.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Rhizobium leguminosarum
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Paramecium tetraurelia
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Blastochloris viridis
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Acinetobacter tartarogenes
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Ralstonia phage phiRSL1
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Opitutus terrae
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells Bradyrhizobium japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
putrescine Vmax: 0.0014 mmol/min/mg Bradyrhizobium japonicum
0.26
-
putrescine Vmax: 0.00132 mmol/min/mg Blastochloris viridis
0.28
-
putrescine Vmax: 0.0088 mmol/min/mg Acinetobacter tartarogenes

Organism

Organism UniProt Comment Textmining
Acinetobacter tartarogenes
-
-
-
Blastochloris viridis
-
-
-
Bradyrhizobium japonicum Q89CN5
-
-
Opitutus terrae B1ZU21
-
-
Paramecium tetraurelia
-
-
-
Ralstonia phage phiRSL1 B2ZXX8
-
-
Rhizobium leguminosarum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 putrescine
-
Rhizobium leguminosarum sym-homospermidine + NH3
-
?
2 putrescine
-
Paramecium tetraurelia sym-homospermidine + NH3
-
?
2 putrescine
-
Blastochloris viridis sym-homospermidine + NH3
-
?
2 putrescine
-
Acinetobacter tartarogenes sym-homospermidine + NH3
-
?
2 putrescine
-
Ralstonia phage phiRSL1 sym-homospermidine + NH3
-
?
2 putrescine
-
Opitutus terrae sym-homospermidine + NH3
-
?
2 putrescine
-
Bradyrhizobium japonicum sym-homospermidine + NH3
-
?

Synonyms

Synonyms Comment Organism
homospermidine synthase orthologue
-
Rhizobium leguminosarum
homospermidine synthase orthologue
-
Paramecium tetraurelia
homospermidine synthase orthologue
-
Blastochloris viridis
homospermidine synthase orthologue
-
Acinetobacter tartarogenes
homospermidine synthase orthologue
-
Ralstonia phage phiRSL1
homospermidine synthase orthologue
-
Opitutus terrae
homospermidine synthase orthologue
-
Bradyrhizobium japonicum
HSS orthologue
-
Rhizobium leguminosarum
HSS orthologue
-
Blastochloris viridis
HSS orthologue
-
Acinetobacter tartarogenes
HSS orthologue
-
Ralstonia phage phiRSL1
HSS orthologue
-
Opitutus terrae
HSS orthologue
-
Bradyrhizobium japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Paramecium tetraurelia
28
-
assay at Blastochloris viridis
28
-
assay at Acinetobacter tartarogenes
28
-
assay at Ralstonia phage phiRSL1
28
-
assay at Opitutus terrae
28
-
assay at Bradyrhizobium japonicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18
-
putrescine
-
Bradyrhizobium japonicum
1.2
-
putrescine
-
Blastochloris viridis
8
-
putrescine
-
Acinetobacter tartarogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
assay at Paramecium tetraurelia
8.4
-
assay at Ralstonia phage phiRSL1
8.4
-
assay at Opitutus terrae
8.4
-
assay at, optimal pH Bradyrhizobium japonicum
8.7
-
assay at, optimal pH Acinetobacter tartarogenes
8.8
-
assay at, optimal pH Blastochloris viridis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhizobium leguminosarum
NAD+
-
Paramecium tetraurelia
NAD+
-
Blastochloris viridis
NAD+
-
Acinetobacter tartarogenes
NAD+
-
Ralstonia phage phiRSL1
NAD+
-
Opitutus terrae
NAD+
-
Bradyrhizobium japonicum

General Information

General Information Comment Organism
malfunction sym-homospermidine is required for normal growth of the alpha-proteobacterium Rhizobium leguminosarum. Symhomospermidine can be replaced, for growth restoration, by the structural analogues spermidine and symnorspermidine, suggesting that the symmetrical or unsymmetrical form, and carbon backbone length are not critical for polyamine function in growth Rhizobium leguminosarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.6
-
putrescine
-
Blastochloris viridis
23.6
-
putrescine
-
Bradyrhizobium japonicum
28.6
-
putrescine
-
Acinetobacter tartarogenes