Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Rhizobium leguminosarum |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Paramecium tetraurelia |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Blastochloris viridis |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Acinetobacter tartarogenes |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Ralstonia phage phiRSL1 |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Opitutus terrae |
expressed in Escherichia coli. The host Escherichia coli cells without the recombinant homospermidine synthase orthologue accumulate putrescine, cadaverine and spermidine and expression of each the homospermidine syntase orthologue in Escherichia coli results in accumulation of homospermidine in the host cells | Bradyrhizobium japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
putrescine | Vmax: 0.0014 mmol/min/mg | Bradyrhizobium japonicum | |
0.26 | - |
putrescine | Vmax: 0.00132 mmol/min/mg | Blastochloris viridis | |
0.28 | - |
putrescine | Vmax: 0.0088 mmol/min/mg | Acinetobacter tartarogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter tartarogenes | - |
- |
- |
Blastochloris viridis | - |
- |
- |
Bradyrhizobium japonicum | Q89CN5 | - |
- |
Opitutus terrae | B1ZU21 | - |
- |
Paramecium tetraurelia | - |
- |
- |
Ralstonia phage phiRSL1 | B2ZXX8 | - |
- |
Rhizobium leguminosarum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 putrescine | - |
Rhizobium leguminosarum | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Paramecium tetraurelia | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Blastochloris viridis | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Acinetobacter tartarogenes | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Ralstonia phage phiRSL1 | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Opitutus terrae | sym-homospermidine + NH3 | - |
? | |
2 putrescine | - |
Bradyrhizobium japonicum | sym-homospermidine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
homospermidine synthase orthologue | - |
Rhizobium leguminosarum |
homospermidine synthase orthologue | - |
Paramecium tetraurelia |
homospermidine synthase orthologue | - |
Blastochloris viridis |
homospermidine synthase orthologue | - |
Acinetobacter tartarogenes |
homospermidine synthase orthologue | - |
Ralstonia phage phiRSL1 |
homospermidine synthase orthologue | - |
Opitutus terrae |
homospermidine synthase orthologue | - |
Bradyrhizobium japonicum |
HSS orthologue | - |
Rhizobium leguminosarum |
HSS orthologue | - |
Blastochloris viridis |
HSS orthologue | - |
Acinetobacter tartarogenes |
HSS orthologue | - |
Ralstonia phage phiRSL1 |
HSS orthologue | - |
Opitutus terrae |
HSS orthologue | - |
Bradyrhizobium japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Paramecium tetraurelia |
28 | - |
assay at | Blastochloris viridis |
28 | - |
assay at | Acinetobacter tartarogenes |
28 | - |
assay at | Ralstonia phage phiRSL1 |
28 | - |
assay at | Opitutus terrae |
28 | - |
assay at | Bradyrhizobium japonicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
putrescine | - |
Bradyrhizobium japonicum | |
1.2 | - |
putrescine | - |
Blastochloris viridis | |
8 | - |
putrescine | - |
Acinetobacter tartarogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Paramecium tetraurelia |
8.4 | - |
assay at | Ralstonia phage phiRSL1 |
8.4 | - |
assay at | Opitutus terrae |
8.4 | - |
assay at, optimal pH | Bradyrhizobium japonicum |
8.7 | - |
assay at, optimal pH | Acinetobacter tartarogenes |
8.8 | - |
assay at, optimal pH | Blastochloris viridis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhizobium leguminosarum | |
NAD+ | - |
Paramecium tetraurelia | |
NAD+ | - |
Blastochloris viridis | |
NAD+ | - |
Acinetobacter tartarogenes | |
NAD+ | - |
Ralstonia phage phiRSL1 | |
NAD+ | - |
Opitutus terrae | |
NAD+ | - |
Bradyrhizobium japonicum |
General Information | Comment | Organism |
---|---|---|
malfunction | sym-homospermidine is required for normal growth of the alpha-proteobacterium Rhizobium leguminosarum. Symhomospermidine can be replaced, for growth restoration, by the structural analogues spermidine and symnorspermidine, suggesting that the symmetrical or unsymmetrical form, and carbon backbone length are not critical for polyamine function in growth | Rhizobium leguminosarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
putrescine | - |
Blastochloris viridis | |
23.6 | - |
putrescine | - |
Bradyrhizobium japonicum | |
28.6 | - |
putrescine | - |
Acinetobacter tartarogenes |