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Literature summary for 2.5.1.47 extracted from
- Purification and characterization of O-acetylserine sulfhydrylase isoenzymes from Datura innoxia (1994), J. Biol. Chem., 269, 6223-6232.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sulfide | above 200 mM | Datura inoxia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | no Michaelis-Menten-kinetics | Datura inoxia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31200 | - |
1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE | Datura inoxia |
| 32000 | - |
2 * 32000, isoenzymes 1 and 3, SDS-PAGE | Datura inoxia |
| 32000 | - |
1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE | Datura inoxia |
| 63000 | - |
isoenzymes one and three, gel filtration | Datura inoxia |
| 86000 | - |
isoenzyme 2, gel filtration | Datura inoxia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + sulfide | Datura inoxia | involved in glutathione formation | L-Cys + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Datura inoxia | - |
three isoenzymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Datura inoxia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Datura inoxia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 880 | - |
isoenzyme 1 and 2 | Datura inoxia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, Tris-HCl, pH 8.1, glycerol, 6 months, no loss of activity | Datura inoxia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-Ser + sulfide | involved in glutathione formation | Datura inoxia | L-Cys + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, isoenzymes 1 and 3, SDS-PAGE | Datura inoxia |
| dimer | 1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE | Datura inoxia |
| More | amino acid composition | Datura inoxia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 58 | all isoenzymes | Datura inoxia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
all isoenzymes | Datura inoxia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | responsible for O-acetylserine binding | Datura inoxia | |
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