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Literature summary for 2.5.1.47 extracted from

  • Burandt, P.; Schmidt, A.; Papenbrock, J.
    Three O-acetyl-L-serine(thiol)lyase isoenzymes from Arabidopsis catalyse cysteine synthesis and cysteine desulfuration at different pH values (2002), J. Plant Physiol., 159, 111-119.
No PubMed abstract available
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzyme A, B and C in Escherichia coli Arabidopsis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0526
-
 cysteine pH 7.5, 37°C, isoenzyme C Arabidopsis sp.
0.0986
-
 cysteine isoenzyme A Arabidopsis sp.
0.113
-
 cysteine pH 7.5, 37°C, isoenzyme B Arabidopsis sp.
0.7
-
 O-acetyl-L-Ser pH 7.5, 37°C, isoenzyme B Arabidopsis sp.
2.1
-
 O-acetyl-L-Ser pH 7.5, 37°C, isoenzyme A Arabidopsis sp.
3.9
-
 O-acetyl-L-Ser pH 7.5, 37°C, isoenzyme C Arabidopsis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isoenzyme A Arabidopsis sp. 5737
-
mitochondrion isoenzyme C Arabidopsis sp. 5739
-
plastid isoenzyme B Arabidopsis sp. 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + dithiothreitol Arabidopsis sp. the side reaction of the enzyme seems to contribute massively to the total H2S release of higher plants at least at higher pH values S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Arabidopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + dithiothreitol
-
 Arabidopsis sp. S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S
-
 ?
L-cysteine + dithiothreitol the side reaction of the enzyme seems to contribute massively to the total H2S release of higher plants at least at higher pH values Arabidopsis sp. S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S
-
 ?
O-acetyl-L-Ser + H2S
-
 Arabidopsis sp. L-Cys + acetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant isoenzyme C Arabidopsis sp.
8
-
 recombinant isoenzyme A and B Arabidopsis sp.