KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-cysteine | pH 7.5, 45°C | Geobacillus stearothermophilus | |
16 | - |
O3-acetyl-L-serine | pH 7.5, 45°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Geobacillus stearothermophilus |
70000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q84IF9 | - |
- |
Purification (Comment) | Organism |
---|---|
ethanol precipitation, ammonium sulfate precipitation, preparative electrophoresis, phenyl sepharose | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + cyanide | - |
Geobacillus stearothermophilus | cyanoalanine + H2S | - |
? | |
additional information | no substrate: L-serine, L-homoserine, O-acetyl-L-homoserine, L-alanine, L-homocysteine | Geobacillus stearothermophilus | ? | - |
? | |
O3-acetyl-L-serine + benzylmercaptan | - |
Geobacillus stearothermophilus | S-benzyl-L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + cyanide | - |
Geobacillus stearothermophilus | beta-cyano-L-alanine + acetate | - |
? | |
O3-acetyl-L-serine + ethylmercaptan | - |
Geobacillus stearothermophilus | S-ethyl-L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + hydrogen sulfide | 970% of the activity with cyanide | Geobacillus stearothermophilus | L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + methylmercaptan | - |
Geobacillus stearothermophilus | S-methyl-L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + phenol | - |
Geobacillus stearothermophilus | O-phenyl-L-serine + acetate | - |
? | |
O3-acetyl-L-serine + phenylmercaptan | - |
Geobacillus stearothermophilus | S-phenyl-L-cysteine + acetate | - |
? | |
O3-acetyl-L-serine + propylmercaptan | - |
Geobacillus stearothermophilus | S-propyl-L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
beta-cyano-L-alanine synthase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, stable in pH-range of 6.0-10.0 | Geobacillus stearothermophilus |
70 | - |
30 min, pH 7.5, stable | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Geobacillus stearothermophilus |