Literature summary for 2.5.1.47 extracted from

Riemenschneider, A.; Riedel, K.; Hoefgen, R.; Papenbrock, J.; Hesse, H.
Impact of reduced O-acetylserine(thiol)lyase isoform contents on potato plant metabolism (2005), Plant Physiol., 137, 892-900.

Cloned(Commentary)

Cloned (Comment)	Organism
to decrease the activity of OASTL, potato plants are transformed with the vector pBinAR harboring a cDNA encoding a sequence from either the StOASTL A or the StOASTL B gene in reverse orientation with respect to the cauliflower mosaic virus 35S promoter. THe transgenic approach is used to downregulate specifically the plastidial and cytosolic isoforms in Solanum tuberosum. This approach results in decreased RNA, protein, and enzymatic activity levels. H2S-releasing capacity is also reduced in these lines. The thiol levels in the transgenic lines are, regardless of the selected OASTL isoform, significantly elevated. Levels of metabolites such as serine, O-acetyl-L-Ser, methionine, threonine, isoleucine, and lysine also increase in the investigated transgenic lines	Solanum tuberosum

Cloned (Comment)

Organism

to decrease the activity of OASTL, potato plants are transformed with the vector pBinAR harboring a cDNA encoding a sequence from either the StOASTL A or the StOASTL B gene in reverse orientation with respect to the cauliflower mosaic virus 35S promoter. THe transgenic approach is used to downregulate specifically the plastidial and cytosolic isoforms in Solanum tuberosum. This approach results in decreased RNA, protein, and enzymatic activity levels. H2S-releasing capacity is also reduced in these lines. The thiol levels in the transgenic lines are, regardless of the selected OASTL isoform, significantly elevated. Levels of metabolites such as serine, O-acetyl-L-Ser, methionine, threonine, isoleucine, and lysine also increase in the investigated transgenic lines

Solanum tuberosum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Solanum tuberosum	9507	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
O-acetyl-L-Ser + H2S	Solanum tuberosum	OASTL activity regulates not only Cys de novo synthesis but also its homeostasis	L-Cys + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Solanum tuberosum	O81154	-	-
Solanum tuberosum	O81155	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Solanum tuberosum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-acetyl-L-Ser + H2S	OASTL activity regulates not only Cys de novo synthesis but also its homeostasis	Solanum tuberosum	L-Cys + acetate	-	?

Synonyms

Synonyms	Comment	Organism
CS-A	-	Solanum tuberosum
CS-B	-	Solanum tuberosum
CSase A	-	Solanum tuberosum
CSase B	-	Solanum tuberosum
OAS-TL A	-	Solanum tuberosum
OAS-TL B	-	Solanum tuberosum
StOASTL A	-	Solanum tuberosum
StOASTL B	-	Solanum tuberosum