Application | Comment | Organism |
---|---|---|
drug development | since mammals lack OASS, the enzyme is a potential target for antimicrobial agents | Salmonella enterica subsp. enterica serovar Typhimurium |
drug development | since mammals lack OASS, the enzyme is a potential target for antimicrobial agents | Haemophilus influenzae |
Cloned (Comment) | Organism |
---|---|
gene cysK, recombinant expression of N-terminal His-tagged enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
gene cysK, recombinant expression of N-terminal His-tagged enzyme | Haemophilus influenzae |
gene cysM, recombinant expression of N-terminal His-tagged enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MNYDI | interaction of the inhibitory pentapeptide MNYDI with CysK OASS isozyme from Salmonella typhimurium, the MNYDI peptide interacts with the HiCysK active site mainly through H-bonds involving its C-terminal carboxylate and hydrophobic interactions involving the side chains of Ile5 and Tyr3, saturation transfer-difference NMR spectroscopy and docking study, docking simulations and molecular modelling, overview | Haemophilus influenzae | |
MNYDI | interaction of the inhibitory pentapeptide MNYDI with CysK OASS isozyme from Salmonella typhimurium, the MNYDI peptide interacts with the HiCysK active site mainly through H-bonds involving its C-terminal carboxylate and hydrophobic interactions involving the side chains of Ile5 and Tyr3, saturation transfer-difference NMR spectroscopy and docking study, docking simulations and molecular modelling, overview; interaction of the inhibitory pentapeptide MNYDI with CysM OASS isozyme from Salmonella typhimurium, saturation transfer-difference NMR spectroscopy and docking study, docking simulations and molecular modelling, overview. In isozyme CysM multiple H-bond interactions are made by Asn2 side chain with S205 side chain and I206 and I209 backbone carbonyl groups | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | anions, like sulfate, significantly reduce the affinity of peptides for CysK | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | - |
additional information | important role of the C-terminal residue Ile5 and the arylic moiety at P3 in dictating enzyme affinity, dissociation constant of MNYDI | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Haemophilus influenzae | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P45040 | CysK; gene cysK | - |
Salmonella enterica subsp. enterica serovar Typhimurium | P0A1E3 | CysK; gene cysK | - |
Salmonella enterica subsp. enterica serovar Typhimurium | P29848 | CysM; gene cysM | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal His-tagged enzyme, cleavage and removal of the tag | Salmonella enterica subsp. enterica serovar Typhimurium |
recombinant N-terminal His-tagged enzyme, cleavage and removal of the tag | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Haemophilus influenzae | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cysK | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
cysK | - |
Haemophilus influenzae |
CysM | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
O-acetylserine sulfhydrylase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
O-acetylserine sulfhydrylase | - |
Haemophilus influenzae |
OASS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
OASS | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
20 | - |
assay at | Haemophilus influenzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
8 | - |
assay at | Haemophilus influenzae |