Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.47 extracted from

  • Mori, M.; Jeelani, G.; Masuda, Y.; Sakai, K.; Tsukui, K.; Waluyo, D.; Tarwadi, D.; Watanabe, Y.; Nonaka, K.; Matsumoto, A.; Omura, S.; Nozaki, T.; Shiomi, K.
    Identification of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites (2015), Front. Microbiol., 6, 962.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.47

Application

Application Comment Organism
drug development the enzyme is a rational drug target against amebiasis Entamoeba histolytica

Cloned(Commentary)

Cloned (Comment) Organism
gene CS1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Entamoeba histolytica
gene CS3, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
aggreticin
-
 Entamoeba histolytica
deacetylkinamycin C
-
 Entamoeba histolytica
deoxyfrenolicin
-
 Entamoeba histolytica
exophillic acid from Exophiala sp.FKI-7082 , specific for isozyme CS1 Entamoeba histolytica
kerriamycin B
-
 Entamoeba histolytica
kerriamycin C
-
 Entamoeba histolytica
additional information identification and evaluation of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites, high-throughput screening. Terreinol and citromycetin are poor inhibitors; identification and evaluation of natural inhibitors of Entamoeba histolytica cysteine synthase from microbial secondary metabolites, high-throughput screening. Terreinol and citromycetin are poor inhibitors, no inhibition of isozyme CS3 by exophillic acid from Exophiala sp.FKI-7082, which is specific for isozyme CS1 Entamoeba histolytica
nanaomycin A
-
 Entamoeba histolytica
naphthacemycin A
-
 Entamoeba histolytica
patulin
-
 Entamoeba histolytica
tetracycline
-
 Entamoeba histolytica
xanthofulvin from Penicillium sp. if08054; from Penicillium sp. if08054 , inhibits isozymes CS1 and CS3 Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide Entamoeba histolytica
-
 L-cysteine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica O15635
-
-
Entamoeba histolytica Q401L7
-
-
no activity in Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide
-
 Entamoeba histolytica L-cysteine + acetate
-
 ?

Synonyms

Synonyms Comment Organism
CS1
-
 Entamoeba histolytica
CS3
-
 Entamoeba histolytica
EhCS1
-
 Entamoeba histolytica
EhCS3
-
 Entamoeba histolytica
O-acetylserine sulfhydrylase
-
 Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Entamoeba histolytica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00031
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica kerriamycin B
0.00056
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica kerriamycin C
0.00063
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica kerriamycin B
0.00064
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica aggreticin
0.00098
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica aggreticin
0.0012
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica kerriamycin C
0.021
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica deacetylkinamycin C
0.022
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica deacetylkinamycin C
0.053
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica nanaomycin A
0.053
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica deoxyfrenolicin
0.057
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica deoxyfrenolicin
0.065
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica nanaomycin A
0.075
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica naphthacemycin A
0.17
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica tetracycline
0.19
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica tetracycline
0.47
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica patulin
0.49
-
 pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica patulin
0.49
-
 above, pH 7.5, 37°C, recombinant enzyme Entamoeba histolytica naphthacemycin A

General Information

General Information Comment Organism
metabolism the enzyme is part of the sulfur assimilatory de novo L-cysteine biosynthetic pathway, that is essential for various cellular activities, including the proliferation and anti-oxidative defense of Entamoeba histolytica Entamoeba histolytica