Cloned (Comment) | Organism |
---|---|
gene OASA1 | Arabidopsis thaliana |
gene OASB1 | Arabidopsis thaliana |
gene OASC1 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a OAS-TL A mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview. The incorporation of sulfur into thiols but not thiol steady-state levels is decreased in mutants lacking cytosolic OAS-TL A | Arabidopsis thaliana |
additional information | generation of a OAS-TL B mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview | Arabidopsis thaliana |
additional information | generation of a OAS-TL C mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview. Lack of mitochondrial OAS-TL C diminishes the incorporation of OAS into thiols without affecting thiol steady-state levels | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme OAS-TL B | Arabidopsis thaliana | 9507 | - |
cytosol | isozyme OAS-TL A | Arabidopsis thaliana | 5829 | - |
mitochondrion | isozyme OAS-TL C | Arabidopsis thaliana | 5739 | - |
additional information | the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. Subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves | Arabidopsis thaliana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Arabidopsis thaliana | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P47998 | gene OASA1 | - |
Arabidopsis thaliana | P47999 | gene OASB1 | - |
Arabidopsis thaliana | Q43725 | gene OASC1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gametophyte | male and female | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
pollen | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Arabidopsis thaliana | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(OAS-TL)-like protein | - |
Arabidopsis thaliana |
cysteine synthase 1 | UniProt | Arabidopsis thaliana |
cysteine synthase, chloroplastic/chromoplastic | UniProt | Arabidopsis thaliana |
cysteine synthase, mitochondrial | UniProt | Arabidopsis thaliana |
O-acetylserine(thiol)lyase | - |
Arabidopsis thaliana |
OAS-TL A | - |
Arabidopsis thaliana |
OAS-TL B | - |
Arabidopsis thaliana |
OAS-TL C | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Cys can be supplied by the mother plant for the development of female gametophytes lacking OAS-TL activity. In contrast, the presence of at least one functional OAS-TL isoform is essential in the male gametophyte. Only the absence of both isozymes OAS-TL A and OAS-TL C results in a decreased incorporation of sulfur and the carbon/nitrogen backbone into thiols, which also causes lower thiol steady-state levels. A segregation pattern can only be explained by a gamete-lethal phenotype of the oastlABC triple mutant. Phenotypes of mutants in the generative phase of the life cycle, overview | Arabidopsis thaliana |
metabolism | the subcellular compartmentation of Cys precursor formation is a remarkable feature of Cys synthesis in higher plants that implies a high degree of regulation between the participating compartments. Contribution of additional OAS-TL-like proteins to Cys synthesis, overview | Arabidopsis thaliana |
physiological function | the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. Subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves | Arabidopsis thaliana |