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Literature summary for 2.5.1.47 extracted from

  • Harun-Ur-Rashid, M.; Oogai, S.; Parveen, S.; Inafuku, M.; Iwasaki, H.; Fukuta, M.; Amzad Hossain, M.; Oku, H.
    Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala (2020), J. Plant Res., 133, 95-108 .
    View publication on PubMed
Search for more literature for 2.5.1.47

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking of substrates Leucaena leucocephala

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 hydrogen sulfide pH 7.0, 40°C Leucaena leucocephala
0.078
-
 hydrogen sulfide pH 7.5, 23°C Leucaena leucocephala
0.148
-
 O-acetyl-L-Ser pH 7.5, 23°C Leucaena leucocephala
0.838
-
 O-acetyl-L-Ser pH 7.0, 40°C Leucaena leucocephala

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Leucaena leucocephala 9507
-
cytosol
-
 Leucaena leucocephala 5829
-

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala
-
-
-
Leucaena leucocephala A0A2Z6G0Q7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme additionally catalyzes synthesis of mimosine, reaction of EC 2.5.1.52. The apparent kcat for Cys production is over sixfold higher than mimosine synthesis and the apparent Km is 3.7 times lower Leucaena leucocephala ?
-
-
additional information enzyme is unable to synthesize mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala ?
-
-
O-acetyl-L-Ser + hydrogen sulfide
-
 Leucaena leucocephala L-Cys + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 34290, calculated from sequence Leucaena leucocephala

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 synthesis of mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala
40
-
-
 Leucaena leucocephala
40
-
 cysteine synthesis Leucaena leucocephala

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.43
-
 hydrogen sulfide pH 7.0, 40°C Leucaena leucocephala
32.65
-
 hydrogen sulfide pH 7.5, 23°C Leucaena leucocephala
43.52
-
 O-acetyl-L-Ser pH 7.5, 23°C Leucaena leucocephala
72.83
-
 O-acetyl-L-Ser pH 7.0, 40°C Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Leucaena leucocephala
7.5
-
 cysteine synthesis Leucaena leucocephala
8
-
 synthesis of mimosine, reaction of EC 2.5.1.52 Leucaena leucocephala

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
 Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Leucaena leucocephala

pI Value

Organism Comment pI Value Maximum pI Value
Leucaena leucocephala calculated from sequence
-
 8.7