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Literature summary for 2.5.1.49 extracted from
- Homocysteine in yeast. Partial purification and properties of O-acetylhomoserine sulfhydrylase (1971), J. Biochem., 70, 1035-1045.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-homoserine | competitive inhibition | Saccharomyces cerevisiae |  |
| L-methionine | competitive inhibition | Saccharomyces cerevisiae | |
| additional information | L-serine, glycine and L-alanine are not inhibitors, dithiothreitol and p-chloromercuribenzoate shows no inhibitory effect | Saccharomyces cerevisiae | |
| O-acetyl-L-serine | competitive inhibition | Saccharomyces cerevisiae | |
| O-succinyl-DL-homoserine | competitive inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.55 | - |
O-acetyl-L-homoserine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 480000 | - |
gel filtration, larger than, no correct value could be estimated | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-homoserine + H2S | Saccharomyces cerevisiae | - |
L-homocysteine + acetic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.2 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | O-acetyl-L-threonine, O-acetyl-L-tyrosine, O-acetyl-L-hydroxyproline and O-succinyl-DL-homoserine are no substrates | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | cystathionine is not synthesized from O-acetylhomoserine and cysteine, O-acetyl-L-serine is no substrate | Saccharomyces cerevisiae | ? | - |
? | |
| O-acetyl-L-homoserine + ethyl mercaptan | - |
Saccharomyces cerevisiae | L-ethionine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + H2S | - |
Saccharomyces cerevisiae | L-homocysteine + acetic acid | - |
? | |
| O-acetyl-L-homoserine + methyl mercaptan | - |
Saccharomyces cerevisiae | L-methionine + acetic acid | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | most stable at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | absolutely required for activity, Km 0.00294 mM | Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
L-methionine | - |
Saccharomyces cerevisiae | |
| 16 | - |
L-homoserine | - |
Saccharomyces cerevisiae | |
| 19 | - |
O-succinyl-DL-homoserine | - |
Saccharomyces cerevisiae | |
| 21.8 | - |
O-acetyl-L-serine | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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