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Literature summary for 2.5.1.54 extracted from
- 2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase (1954), J. Biol. Chem., 234, 716-722.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-deoxy-D-arabino-heptonic acid 7-phosphate | - |
Escherichia coli | |
| D-fructose 1,6-diphosphate | - |
Escherichia coli | |
| D-sedoheptulose 1,7-diphosphate | - |
Escherichia coli | |
| D-sedoheptulose 7-phosphate | - |
Escherichia coli | |
| p-chloromercuribenzoate | complete inhibition at 0.02 mM, reversible by cysteine | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-erythrose 4-phosphate | pH 6.4, 37°C | Escherichia coli | |
| 3.5 | - |
phosphoenolpyruvate | pH 6.4, 37°C | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
mutant 83-24 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
partially purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, ammonium sulfate and acetone fractions, stable for at least several weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with D-erythrose, D-glyceraldehyde 3-phosphate, ribose 5-phosphate, glucose 6-phosphate, glucosamine 6-phosphate, N-acetylglucosamine 6-phosphate, and pyruvate | Escherichia coli | ? | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Escherichia coli |
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