Cloned (Comment) | Organism |
---|---|
expression of enzyme with affinity purification tagwith plasmid pKTDS-HN in Escherichia coli KA13 | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
in complex with chorismate mutase, hanging-drop method, in 20 mM BTP, pH 7.5, 150 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine hydrochloride, 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, crystallization after 2 months with no ammonium sulfate and 0.1 M Tris-HCl, pH 7.9-8.0 and PEG 400 or glycerol | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51830 | - |
expected mass, calculated from the 472 amino acids | Mycobacterium tuberculosis |
51830 | - |
observed by ESI-MS | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Mycobacterium tuberculosis | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | O53512 | - |
- |
Purification (Comment) | Organism |
---|---|
cells centrifuged and resuspended in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP (tris(2-carboxyethyl)phosphine hydrochloride), 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, cell lysis with lysozyme and sonication, centrifugation, crude extract subjected to Ni-NTA agarose chromatography, elution with 250 mM imidazole in buffer, further purification by preparative FPLC HiLoad 26/60 Superdex 75 gel-filtration column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Mycobacterium tuberculosis | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-7-phosphoheptulonate synthase | - |
Mycobacterium tuberculosis |
DAHP synthase | - |
Mycobacterium tuberculosis |
MtDS | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | initial step in the shikimate biosynthesis pathway, enhances the catalytic efficiency of chorismate mutase in a noncovalent enzyme complex | Mycobacterium tuberculosis |