Cloned (Comment) | Organism |
---|---|
gene aroG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain (DE3) Star | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
H29A | site-directed mutagenesis, the mutant is inhibited by both L-Tyr and L-Phe | Thermotoga maritima |
H29S/S31H | site-directed mutagenesis, the mutant is inhibited to a greater extent by L-Phe than L-Tyr | Thermotoga maritima |
S31G | site-directed mutagenesis, the mutation severely reduces inhibition by L-Tyr | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | involved in allosteric regulation of the enzyme, inhibits the wild-type enzyme, and enzyme mutants H29A and H29S/S31H | Thermotoga maritima | |
L-tyrosine | allosteric regulation of the enzyme is mediated by L-Tyr binding to a discrete ACT regulatory domain appended to a core catalytic (beta/alpha)8 barrel, highly reduced inhibition of enzyme mutant S31G. Comparison of the position of the regulatory ACT domain of TmaDAH7PS in the unbound and L-Tyr-bound conformations, overview | Thermotoga maritima | |
additional information | small angle X-ray (SAXS) scattering analysis | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Thermotoga maritima | |
0.00485 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima | |
0.0128 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima | |
0.013 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima | |
0.021 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
0.028 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima | |
0.03 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
0.033 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima | |
0.039 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Thermotoga maritima | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYH8 | gene aroF | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Thermotoga maritima | 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase | - |
Thermotoga maritima |
DAH7PS | - |
Thermotoga maritima |
phospho-2-dehydro-3-deoxyheptonate aldolase | UniProt | Thermotoga maritima |
TmaDAH7PS | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.7 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima | |
11.7 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima | |
14.1 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima | |
14.1 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima | |
14.3 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
14.3 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima | |
17.5 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
17.5 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the first step of the shikimate pathway for the biosynthesis of aromatic amino acids | Thermotoga maritima |
physiological function | allosteric regulation of the enzyme is mediated by L-Tyr binding to a discrete ACT regulatory domain appended to a core catalytic (beta/alpha)8 barrel | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
360 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima | |
430 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima | |
500 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29S/S31H | Thermotoga maritima | |
580 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
700 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant H29A | Thermotoga maritima | |
900 | - |
D-erythrose 4-phosphate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima | |
1400 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, mutant S31G | Thermotoga maritima | |
2400 | - |
phosphoenolpyruvate | pH 7.3, temperature not specified in the publication, wild-type enzyme | Thermotoga maritima |