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Literature summary for 2.5.1.55 extracted from

  • Radaev, S.; Dastidar, P.; Patel, M.; Woodard, R.W.; Gatti, D.L.
    Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (2000), J. Biol. Chem., 275, 9476-9484.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization is performed by vapor diffusion in hanging drops Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-arabinose-5-phosphate + H2O Escherichia coli 2-dehydro-3-deoxy-D-octonate 8-phosphate is the phosphorylated precursor of 3-deoxy-D-manno-octulosonate, an essential sugar of the liposaccharide of gram-negative bacteria 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
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Organism

Organism UniProt Comment Textmining
Escherichia coli P0A715
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-

Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate the synthesis of 2-dehydro-3-deoxy-D-octonate 8-phosphate proceeds through the formation of a linear rather than a cyclic intermediate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate
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Escherichia coli 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
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?
phosphoenolpyruvate + D-arabinose-5-phosphate + H2O 2-dehydro-3-deoxy-D-octonate 8-phosphate is the phosphorylated precursor of 3-deoxy-D-manno-octulosonate, an essential sugar of the liposaccharide of gram-negative bacteria Escherichia coli 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
tetramer the enzyme is a homotetramer in which each monomer has the fold of a (beta/alpha)8 barrel Escherichia coli