Application | Comment | Organism |
---|---|---|
medicine | enzyme absent in mammals, therefore potential target for the development of new antibiotics | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 4 Cd2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer, Cd2+ reconstituted enzyme is less stable than that of Zn2+, Co2+ and Cu2+ enzymes | Helicobacter pylori | |
Co2+ | monomer and dimer are each bound with 1 metal ion, tetramer with 2 | Helicobacter pylori | |
Cu2+ | 4 Cu2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer | Helicobacter pylori | |
additional information | divalent metal ions play an important role in the quaternary structure of the protein | Helicobacter pylori | |
Zn2+ | 4 Zn2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30400 | - |
monomer with 1 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
30440 | - |
monomer with 1 Zn2+ or 1 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
30480 | - |
monomer with 1 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
60690 | - |
dimer with 1 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
60760 | - |
dimer with 1 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
60840 | - |
dimer with 2 Zn2+ or 2 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
121500 | - |
tetramer with 2 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
121600 | - |
tetramer with 4 Zn2+ or 4 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
121800 | - |
tetramer with 4 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Helicobacter pylori | - |
2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
apoenzyme + 200 microM metal ion salt + 50 mM Tris-HCl buffer, pH 7.5 dialyzed against 50 mM ammonium acetate buffer, pH 7.5, 4°C | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Helicobacter pylori | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30440 (with 2 Co2+), 4 * 30440 (with 4 Zn2+ or Cu2+), 4 * 30480 (with 4 Cd2+), tetramer formed by 2 dimers each, ESI-MS | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase | - |
Helicobacter pylori |
Kdo8P synthase | - |
Helicobacter pylori |