Cloned (Comment) | Organism |
---|---|
gene kdo8ps, recombinant expression of His6-tagged codon-optimized enzyme in Escherichia coli strain Rosetta2 (pLysS) DE3 | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.003 ml of 5 mg/ml protein in 0.2 M Tris-HCl, pH 7.4, 0.1 M NaCl, 0.05% 2-mercaptoethanol, and 0.1 mM phosphoenolpyruvate with 0.0015 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% w/v PEG 8000, 20°C, 2 days, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacementusing structure PDB ID 1g7v and model building | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Pseudomonas aeruginosa | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene kdo8ps | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Pseudomonas aeruginosa | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | two-dimensional structure comparison | Pseudomonas aeruginosa |
oligomer | the enzyme structure is a prototypical alpha/beta TIM barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
2-dehydro-3-deoxyphosphooctonate aldolase | - |
Pseudomonas aeruginosa |
2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase | - |
Pseudomonas aeruginosa |
KDO8PS | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
additional information | active site structure, overview | Pseudomonas aeruginosa |
physiological function | the enzyme catalyzes a key step in the synthesis of the lipopolysaccharide of most Gram-negative bacteria, it catalyzes the condensation reaction between phosphoenolpyruvate and arabinose 5-phosphate to produce 3-deoxy-D-manno-octulosonate 8-phosphate. The enzyme is vital for for proper synthesis and assembly of lipoplysaccharides and the survival and virulence of Pseudomonas aeruginosa | Pseudomonas aeruginosa |