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Literature summary for 2.5.1.55 extracted from
- Heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of KDO8P synthase from Arabidopsis thaliana (2014), Protein Expr. Purif., 101, 133-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene kdsA1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Arabidopsis thaliana |
| gene kdsA2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme, hanging-drop vapor diffusion method, mixing of 300 nl of 10 mg/ml protein in 30 mM Tris-HCl, pH 8.0, 200 mM NaCl with 300 nl reservoir solution containing 28% v/v PEG 400, 0.1 M HEPES, pH 7.5, and 0.2 M CaCl2, X-ray diffraction structure determinatin and analysis | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33500 | - |
- |
Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Arabidopsis thaliana | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q6NQL4 | gene kdsA2; gene kdsA2 | - |
| Arabidopsis thaliana | Q9AV97 | gene kdsA1; gene kdsA1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Arabidopsis thaliana | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33545, sequence calculation, 2 * 33500, recombinant His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate synthase | - |
Arabidopsis thaliana |
| 3-eoxy-D-manno-octulosonate 8-phosphate synthase | - |
Arabidopsis thaliana |
| KDO8PS | - |
Arabidopsis thaliana |
| KdsA1 | - |
Arabidopsis thaliana |
| KdsA2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arabidopsis thaliana |
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