Application | Comment | Organism |
---|---|---|
medicine | evidence that inhibitors of enzyme could be effective therapeutic agents in treatment of many human cancers | Rattus norvegicus |
medicine | prime target for development of anticancer therapeutics | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure, modelling indicates that geranylgeranyl diphosphate adopts a different conformation than the farnesyl chain of farnesyl diphosphate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | correlation between Km and analogue hydrophobicity and length | Rattus norvegicus | |
0.046 | - |
farnesyl diphosphate | - |
Rattus norvegicus | |
0.056 | - |
8-benzyloxy-3-methyl-2-octen-1-ol-diphosphate | - |
Rattus norvegicus | |
0.089 | - |
10-benzyloxy-3-methyl-2-decen-1-ol-diphosphate | - |
Rattus norvegicus | |
0.091 | - |
9-benzyloxy-3-methyl-2-nonen-1-ol-diphosphate | - |
Rattus norvegicus | |
0.13 | - |
7-benzyloxy-3-methyl-2-hepten-1-ol-diphosphate | - |
Rattus norvegicus | |
0.45 | - |
geranylgeranyl diphosphate | - |
Rattus norvegicus | |
0.89 | - |
6-benzyloxy-3-methyl-2-hexen-1-ol-diphosphate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus | |
Mg2+ | appears to coordinate the diphosphate moiety of farnesyl diphosphate | Rattus norvegicus | |
Zn2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | process required for the transforming activity of oncogenic variants of Ras, making enzyme a prime target for anticancer therapeutics | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | process necessary for the subcellular localisation of substrate to the plasma membrane | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | enzyme responsible for catalysing isoprene lipid modifications | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | Rattus norvegicus | A series of steps modifies the C-terminus of Ras. Enzyme catalyses the first of these steps by transferring the farnesyl group from farnesyl diphosphates to the cysteine side chain in the CAAX sequence motif at the C-terminus of Ras | S-farnesyl protein + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate | kinetic mechanism | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-benzyloxy-3-methyl-2-decen-1-ol-diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-(10-benzyloxy-3-methyl-2-decenyl)-Cys-Val-Leu-Ser | - |
? | |
6-benzyloxy-3-methyl-2-hexen-1-ol-diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-(6-benzyloxy-3-methyl-2-hexenyl)-Cys-Val-Leu-Ser | - |
? | |
7-benzyloxy-3-methyl-2-hepten-1-ol-diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-(7-benzyloxy-3-methyl-2-heptenyl)-Cys-Val-Leu-Ser | - |
? | |
8-benzyloxy-3-methyl-2-octen-1-ol-diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-(8-benzyloxy-3-methyl-2-octenyl)-Cys-Val-Leu-Ser | - |
? | |
9-benzyloxy-3-methyl-2-nonen-1-ol-diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-(9-benzyloxy-3-methyl-2-nonenyl)-Cys-Val-Leu-Ser | - |
? | |
farnesyl diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | the best substrate | Rattus norvegicus | diphosphate + N-dansyl-Gly-S-farnesyl-Cys-Val-Leu-Ser | - |
? | |
farnesyl diphosphate + protein-cysteine | posttranslational lipid modification in which a 15-carbon farnesyl isoprenoid is linked via a thioether bond to specific cysteine residues of proteins, the reactive cysteine is located in the C-terminal Ca1a2X motif in which C is the modified cysteine, a1 and a2 are often an aliphatic residue, and X is Ser, Met, Ala or Gln | Rattus norvegicus | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | process required for the transforming activity of oncogenic variants of Ras, making enzyme a prime target for anticancer therapeutics | Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | process necessary for the subcellular localisation of substrate to the plasma membrane | Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | enzyme responsible for catalysing isoprene lipid modifications | Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + protein-cysteine | A series of steps modifies the C-terminus of Ras. Enzyme catalyses the first of these steps by transferring the farnesyl group from farnesyl diphosphates to the cysteine side chain in the CAAX sequence motif at the C-terminus of Ras | Rattus norvegicus | S-farnesyl protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + N-dansyl-Gly-Cys-Val-Leu-Ser | - |
Rattus norvegicus | diphosphate + N-dansyl-Gly-S-geranylgeranyl-Cys-Val-Leu-Ser | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | - |
Rattus norvegicus | diphosphate + S-geranylgeranylcysteinyl protein | - |
? | |
additional information | not: geranylgeranyl diphosphate | Rattus norvegicus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
7-benzyloxy-3-methyl-2-hepten-1-ol-diphosphate | - |
Rattus norvegicus | |
15 | - |
geranylgeranyl diphosphate | - |
Rattus norvegicus | |
25 | - |
10-benzyloxy-3-methyl-2-decen-1-ol-diphosphate | - |
Rattus norvegicus | |
27 | - |
8-benzyloxy-3-methyl-2-octen-1-ol-diphosphate | - |
Rattus norvegicus | |
43 | - |
9-benzyloxy-3-methyl-2-nonen-1-ol-diphosphate | - |
Rattus norvegicus | |
44 | - |
6-benzyloxy-3-methyl-2-hexen-1-ol-diphosphate | - |
Rattus norvegicus | |
100 | - |
farnesyl diphosphate | - |
Rattus norvegicus |