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Literature summary for 2.5.1.6 extracted from

  • Schroeder, G.; Eichel, J.; Breinig, S.; Schroeder, J.
    Three differentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: molecular and functional characterization (1997), Plant Mol. Biol., 33, 211-222.
    View publication on PubMed
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
three isoenzymes expressed in Escherichia coli Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
5-amino-L-norvaline 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM Catharanthus roseus
ADP 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM Catharanthus roseus
CTP 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM; 60-70% inhibition with 5 mM Catharanthus roseus
Ethionine 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM Catharanthus roseus
GTP not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM; not accepted as a substrate but inhibits the reaction in the presence of ATP, 70-80% inhibition with 5 mM Catharanthus roseus
additional information addition of reducing agents has no effect; addition of reducing agents has no effect; addition of reducing agents has no effect Catharanthus roseus
putrescine 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM Catharanthus roseus
spermidine 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM Catharanthus roseus
spermine 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM Catharanthus roseus
tripolyphosphate strong inhibitor; strong inhibitor; strong inhibitor Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 L-methionine
-
 Catharanthus roseus
0.18
-
 ATP
-
 Catharanthus roseus
0.25
-
 ATP
-
 Catharanthus roseus
0.31
-
 ATP
-
 Catharanthus roseus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cations required for optimal activity, 50 mM K+ sufficient for full activity, can be replaced by NH4+ but not by Na+ Catharanthus roseus
Mg2+ strictly dependent on divalent cations with maximum activity at 5mM and Mg2+ fully replaceable by Mn2+ or Co2+ salts Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42560
-
 SAMS 3, gel filtration Catharanthus roseus
43000
-
 SAMS 2, gel filtration Catharanthus roseus
43050
-
 SAMS 1, gel filtration Catharanthus roseus

Organism

Organism UniProt Comment Textmining
Catharanthus roseus Q96551 SAMS1; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-
Catharanthus roseus Q96552 SAMS2; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-
Catharanthus roseus Q96553 SAMS3; Madagascar perwinkle, L. G. Don, line CP3a, three isoenzymes: SAMS 1, 2 and 3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
 Catharanthus roseus S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Catharanthus roseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Catharanthus roseus
37 45
-
 Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.3 three isoenzymes Catharanthus roseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
 tripolyphosphate
-
 Catharanthus roseus