Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | tripolyphosphatase activity stimulated by preincubation with ATP and methionine | Rattus norvegicus | |
methionine | stimulation of activity demonstrated in vivo. Tripolyphosphatase activity stimulated by preincubation with ATP and methionine | Rattus norvegicus | |
additional information | no effect with ADP and methionine or S-adenosylmethionine | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP and methionine act as a switch between two different MAT III isoforms | Rattus norvegicus | |
L-methionine | ATP and methionine act as a switch between two different MAT III isoforms | Rattus norvegicus | |
nitric oxide | two MAT III isoforms, one with low tripolyphosphatase activity that is insensitive to NO and another with high tripolyphosphatase activity that is inhibited by NO | Rattus norvegicus | |
S-nitrosoglutathione | inhibits S-adenosylmethionine sinthetase activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Rattus norvegicus | two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
two MAT III isoforms | - |
Purification (Comment) | Organism |
---|---|
method that includes DEAE-Sepharose, phenyl-Sepharose and blue-Sepharose chromatographies and ultrafiltration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | mechanism | Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction | Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
tripolyphosphate + H2O | - |
Rattus norvegicus | diphosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
tripolyphosphate | tripolyphosphatase activity | Rattus norvegicus | |
0.583 | - |
S-adenosylmethionine | S-adenosylmethionine synthesis | Rattus norvegicus |