Protein Variants | Comment | Organism |
---|---|---|
D121N | no basal enzymic activity, little activity in presence of S-adenosyl methionine | Leishmania donovani |
D166N | reduced enzymic activity, little activation by S-adenosyl methionine | Leishmania donovani |
D19N | no basal enzymic activity, little activity in presence of S-adenosyl methionine | Leishmania donovani |
D249N | reduced enzymic activity, little activation by S-adenosyl methionine | Leishmania donovani |
D277N | no enzymic activity | Leishmania donovani |
D282N | reduced enzymic activity, little activation by S-adenosyl methionine | Leishmania donovani |
F241A | no enzymic activity, but hydrolysis of triphosphate | Leishmania donovani |
H17A | no basal enzymic activity, little activity in presence of S-adenosyl methionine | Leishmania donovani |
H17N | no basal enzymic activity, little activity in presence of S-adenosyl methionine | Leishmania donovani |
K168A | no enzymic activity | Leishmania donovani |
K256A | no activation by S-adenosyl methionine | Leishmania donovani |
K276A | no enzymic activity | Leishmania donovani |
K280A | enhanced enzymic activity, little activation by S-adenosyl methionine | Leishmania donovani |
R255L | no enzymic activity | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-methionine | mutant D249N, pH 8.0 | Leishmania donovani | |
0.036 | - |
L-methionine | mutant D166N, pH 8.0 | Leishmania donovani | |
0.038 | - |
L-methionine | mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.04 | - |
L-methionine | wild-type, pH 8.0 | Leishmania donovani | |
0.041 | - |
L-methionine | mutant K280A, pH 8.0 | Leishmania donovani | |
0.045 | - |
L-methionine | mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.05 | - |
L-methionine | mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.074 | - |
L-methionine | mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.137 | - |
L-methionine | mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.137 | - |
L-methionine | mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.141 | - |
L-methionine | mutant D282N, pH 8.0 | Leishmania donovani | |
0.147 | - |
L-methionine | mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.17 | - |
L-methionine | mutant K256A, pH 8.0 | Leishmania donovani | |
0.17 | - |
L-methionine | mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.176 | - |
L-methionine | mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani | |
0.287 | - |
L-methionine | wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q9U6Q8 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66 | - |
mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
108 | - |
mutant D166N, pH 8.0 | Leishmania donovani |
108 | - |
mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
108 | - |
mutant D282N, pH 8.0 | Leishmania donovani |
138 | - |
mutant D249N, pH 8.0 | Leishmania donovani |
210 | - |
mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
222 | - |
wild-type, pH 8.0 | Leishmania donovani |
240 | - |
mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
252 | - |
mutant K256A, pH 8.0 | Leishmania donovani |
252 | - |
mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
318 | - |
mutant K280A, pH 8.0 | Leishmania donovani |
330 | - |
mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
720 | - |
mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
792 | - |
mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
960 | - |
mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
3000 | - |
wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Leishmania donovani | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |