Protein Variants | Comment | Organism |
---|---|---|
C35S | reduction in Vmax value | Rattus norvegicus |
C61S | reduction in Vmax value | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.222 | - |
methionine | mutant C35S, presence of dithiothreitol | Rattus norvegicus | |
0.246 | - |
methionine | wild-type, presence of dithiothreitol | Rattus norvegicus | |
0.449 | - |
methionine | mutant C35S, presence of glutathione | Rattus norvegicus | |
0.493 | - |
ATP | mutant C35S, presence of dithiothreitol | Rattus norvegicus | |
0.53 | - |
ATP | wild-type, presence of glutathione | Rattus norvegicus | |
0.588 | - |
ATP | wild-type, presence of dithiothreitol | Rattus norvegicus | |
0.756 | - |
methionine | mutant C61S, presence of dithiothreitol | Rattus norvegicus | |
0.778 | - |
ATP | mutant C35S, presence of glutathione | Rattus norvegicus | |
0.794 | - |
methionine | mutant C61S, presence of glutathione | Rattus norvegicus | |
1.12 | - |
methionine | wild-type, presence of glutathione | Rattus norvegicus | |
2.54 | - |
ATP | mutant C61S, presence of glutathione | Rattus norvegicus | |
3.25 | - |
ATP | mutant C61S, presence of dithiothreitol | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44300 | - |
2 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
44300 | - |
4 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
92700 | - |
gel filtration | Rattus norvegicus |
194900 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P13444 | recombinant enzyme, isoform MAT I, tetramer, and MAT III, dimer | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31500 | - |
mutant C61S, presence of glutathione | Rattus norvegicus |
32700 | - |
mutant C35S, presence of glutathione | Rattus norvegicus |
69070 | - |
mutant C61S, presence of dithiothreitol | Rattus norvegicus |
94470 | - |
mutant C35S, presence of dithiothreitol | Rattus norvegicus |
95800 | - |
wild-type, presence of glutathione | Rattus norvegicus |
110000 | - |
wild-type, presence of dithiothreitol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Rattus norvegicus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
tetramer | 4 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |