Protein Variants | Comment | Organism |
---|---|---|
additional information | a single mutant metK10 with one nucleotide substitution in the metK gene resulting in a 15fold decrease in SAM synthetase activity and a 4fold decrease in SAM concentration in vivo, the metK10 mutation specifically affects S-box gene expression, and the increase in expression under repressing conditions is dependent on the presence of a functional transcriptional antiterminator element, phenotype, overview | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Bacillus subtilis | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
additional information | Bacillus subtilis | genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene metK, strain ZB307Spc | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Bacillus subtilis | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
additional information | genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S-adenosylmethionine synthetase | - |
Bacillus subtilis |
SAM synthetase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |