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Literature summary for 2.5.1.6 extracted from

  • McDaniel, B.A.; Grundy, F.J.; Kurlekar, V.P.; Tomsic, J.; Henkin, T.M.
    Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression (2006), J. Bacteriol., 188, 3674-3681.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information a single mutant metK10 with one nucleotide substitution in the metK gene resulting in a 15fold decrease in SAM synthetase activity and a 4fold decrease in SAM concentration in vivo, the metK10 mutation specifically affects S-box gene expression, and the increase in expression under repressing conditions is dependent on the presence of a functional transcriptional antiterminator element, phenotype, overview Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Bacillus subtilis
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?
additional information Bacillus subtilis genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene metK, strain ZB307Spc
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Bacillus subtilis phosphate + diphosphate + S-adenosyl-L-methionine
-
?
additional information genes in the S-box family are regulated by binding of S-adenosylmethionine to the 5' region of the mRNA of the regulated gene, SAM binding promotes a rearrangement of the RNA structure that results in premature termination of transcription in vitro and repression of expression of the downstream coding sequence, the S-box RNA element therefore acts as a SAM-binding riboswitch in vitro Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
S-adenosylmethionine synthetase
-
Bacillus subtilis
SAM synthetase
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis