Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
the Ni2+-IDA agarose-immobilized synthetase exhibits 40.4% of the free enzyme activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | causes complete inactivation of the enzyme | Saccharomyces cerevisiae | |
ATP | causes complete inactivation of the enzyme | Saccharomyces cerevisiae | |
Ba2+ | 70.70% residual activity at 5 mM | Saccharomyces cerevisiae | |
Br- | 93.33% residual activity at 5 mM | Saccharomyces cerevisiae | |
Ca2+ | 86.36% residual activity at 5 mM | Saccharomyces cerevisiae | |
CH3COO- | 92.25% residual activity at 5 mM | Saccharomyces cerevisiae | |
Cl- | 85.27% residual activity at 5 mM | Saccharomyces cerevisiae | |
Cu2+ | 25.74% residual activity at 5 mM | Saccharomyces cerevisiae | |
F- | 88.84% residual activity at 5 mM | Saccharomyces cerevisiae | |
Fe2+ | 59.22% residual activity at 5 mM | Saccharomyces cerevisiae | |
GSH | causes complete inactivation of the enzyme | Saccharomyces cerevisiae | |
I- | 87.91% residual activity at 5 mM | Saccharomyces cerevisiae | |
K+ | 85.27% residual activity at 5 mM | Saccharomyces cerevisiae | |
Li+ | 81.40% residual activity at 5 mM | Saccharomyces cerevisiae | |
Mn2+ | 73.49% residual activity at 5 mM | Saccharomyces cerevisiae | |
additional information | no effect on activity at 0.1 mM Ni2+ | Saccharomyces cerevisiae | |
Na+ | 80.16% residual activity at 5 mM | Saccharomyces cerevisiae | |
Zn2+ | 22.17% residual activity at 5 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.527 | - |
L-methionine | at pH 8.0 and 35°C | Saccharomyces cerevisiae | |
0.631 | - |
ATP | at pH 8.0 and 35°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, H2PO4-, NH4-, and NO3- have no significant effect on enzyme activity at 5 mM | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P19358 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-IDA agarose column chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.42 | - |
crude extract, at 35°C | Saccharomyces cerevisiae |
23.83 | - |
after 3.71fold purification, at 35°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Saccharomyces cerevisiae | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP: L-methionine S-adenosyltransferase | - |
Saccharomyces cerevisiae |
S-adenosylmethionine synthetase | - |
Saccharomyces cerevisiae |
SAM synthetase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | enzyme activity is significantly reduced at temperatures below 30°C or above 40°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | the activity of the enzyme is decreased above pH 9.0 and is inactive at pH below 7.5 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |