Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
sitting drop method at 20°C, crystallization of the enzyme in the S-adenosylmethionine bound, S-adenosylethionine bound and unbound forms | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
L-methionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
0.0028 | - |
L-methionine | pH 8.0, 65°C | Saccharolobus solfataricus | |
0.0056 | - |
L-ethionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
0.007 | - |
L-ethionine | pH 8.0, 65°C | Saccharolobus solfataricus | |
0.0145 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
0.0592 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-ethionine | Saccharolobus solfataricus | |
0.0686 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
0.0969 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-ethionine | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980S9 | - |
- |
Saccharolobus solfataricus P2 | Q980S9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O | 10% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O | 10% of the turnover rate compared to L-methionine | Saccharolobus solfataricus P2 | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O | 30% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O | 30% of the turnover rate compared to L-methionine | Saccharolobus solfataricus P2 | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O | 70% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O | 70% of the turnover rate compared to L-methionine | Saccharolobus solfataricus P2 | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(butylsulfanyl)butanoic acid + H2O | 27% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(butylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(ethylselanyl)butanoic acid + H2O | 76% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(ethylsulfanyl)butanoic acid + H2O | 84% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(ethylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid + H2O | 30% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid + H2O | 27% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid + H2O | 28% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid + H2O | 36% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid + H2O | 40% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid + H2O | 53% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(propylselanyl)butanoic acid + H2O | 66% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propylselanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-(propylsulfanyl)butanoic acid + H2O | 44% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propylsulfanyl)butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid + H2O | 50% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2-methyl propyl)sulfanyl] butanoic acid + H2O | 57% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methyl propyl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid + H2O | 46% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid + H2O | 15% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid + H2O | 27% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid + H2O | 25% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid + H2O | 60% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(3-methyl butyl)sulfanyl]butanoic acid + H2O | 36% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methyl butyl)sulfanyl] butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid + H2O | 44% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid + H2O | 12% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid + H2O | 68% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl)sulfanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid + H2O | 12% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid + H2O | 38% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid + H2O | 24% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid | - |
? | |
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid + H2O | 28% of the turnover rate compared to L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid | - |
? | |
ATP + L-ethionine + H2O | - |
Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-L-ethionine | - |
? | |
ATP + L-methionine + H2O | - |
Saccharolobus solfataricus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | - |
Saccharolobus solfataricus P2 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-selenomethionine + H2O | as active as L-methionine | Saccharolobus solfataricus | phosphate + diphosphate + Se-adenosyl-L-selenomethionine | - |
? | |
additional information | the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSO0199 | locus name | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0328 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
0.0343 | - |
L-methionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
0.039 | - |
L-ethionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
0.041 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-ethionine | Saccharolobus solfataricus | |
0.047 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
0.049 | - |
L-methionine | pH 8.0, 65°C | Saccharolobus solfataricus | |
0.05 | - |
L-ethionine | pH 8.0, 65°C | Saccharolobus solfataricus | |
0.052 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-ethionine | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-ethionine | Saccharolobus solfataricus | |
0.48 | - |
ATP | pH 8.0, 37°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
0.87 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-ethionine | Saccharolobus solfataricus | |
3.23 | - |
ATP | pH 8.0, 65°C, cosubstrate: L-methionine | Saccharolobus solfataricus | |
6.95 | - |
L-ethionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
7.12 | - |
L-ethionine | pH 8.0, 65°C | Saccharolobus solfataricus | |
14.92 | - |
L-methionine | pH 8.0, 37°C | Saccharolobus solfataricus | |
17.37 | - |
L-methionine | pH 8.0, 65°C | Saccharolobus solfataricus |