Protein Variants | Comment | Organism |
---|---|---|
additional information | homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%) and result in unprenylated RAB GTPase accumulation in the cytoplasm, up-regulation (50% and more) of about one third of RAB-GTPase paralogs in dark-grown plants compared to light-grown plants, down-regulation of about one tenth, endocytosis and exocytosis are downregulated in homozygotes defective in shoot growth and morphogenesis, root gravitropism is normal but compromised in shoots, etiolation is defective, mutants show constitutive photomorphogenic phenotypes, smaller rosette diameter, loss of apical dominance | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein | Arabidopsis thaliana | homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min | S-geranylgeranyl-RAB GTPase + diphosphate | - |
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Organism | UniProt | Comment | Textmining |
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Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
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fresh plant material is frozen in liquid nitrogen, ground on ice with acid-washed sand in 100 mM Tris buffer, pH 7.8, containing 5 mM EGTA, 5 mM EDTA, 10 mM beta-mercaptoethanol, and 10% glycerol, lysate is centrifuged, dialyzed against 50 mM Tris buffer, pH 7.8, containing 10% glycerol, and 1 mM DTT | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
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gametophyte | both beta subunit levels are similar in male gametophytes, subunit A1 is expressed in all tissues, subunit A2 is expressed significantly only in pollen | Arabidopsis thaliana | - |
sporophyte | subunit B1 is about 10times more abundant in sporophytes than subunit B2, subunit A1 is expressed in all tissues | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein | homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min | Arabidopsis thaliana | S-geranylgeranyl-RAB GTPase + diphosphate | - |
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Synonyms | Comment | Organism |
---|---|---|
Rab geranylgeranyltransferase type II | - |
Arabidopsis thaliana |
RAB-GGT | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | homozygous mutants of the major beta-subunit of Arabidopsis enzyme (RGTB1) result in unprenylated RAB GTPase accumulation in the cytoplasm, up-regulation of several GTPases, endocytosis and exocytosis are downregulated, defects in shoot growth and morphogenesis, changed shoot gravitropism and reaction to etiolation | Arabidopsis thaliana |
physiological function | membrane localization of RAB GTPase is mediated by C-terminal double geranylgeranylation by the enzyme in cooperation with the RAB escort protein (REP) | Arabidopsis thaliana |