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Literature summary for 2.5.1.61 extracted from

  • Anderson, P.M.; Desnick, R.J.
    Purification and properties of uroporphyrinogen I synthase from human erythrocytes (1980), J. Biol. Chem., 255, 1993-1999.
    View publication on PubMed
Search for more literature for 2.5.1.61

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.1 mM CaCl2, 80% inhibition Homo sapiens
Cu2+ 1 mM CuSO4, 87% inhibition Homo sapiens
Fe2+ 1 mM FeSO4, 58% inhibition Homo sapiens
Fe3+ 1 mM FeCl3, 62% inhibition Homo sapiens
Hg2+ 0.0004 mM HgCl2, 80% inhibition Homo sapiens
Mg2+ 50 mM MgCl2, 55% inhibition Homo sapiens
N-ethylmaleimide 1 mM 38% inhibition Homo sapiens
p-chloromercuribenzoate 0.002 mM, 74% inhibition Homo sapiens
Pb(NO3)2 0.005 mM, 35% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 porphobilinogen A and B form Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration, A form Homo sapiens
37000
-
 1 * 37000, SDS-PAGE, A and B form Homo sapiens
38000
-
 gel filtration, B form Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens occurrence of multiple forms of the enzyme. These isomers correspond to the enzyme-substrate intermediates: mono-, di-, tri-, and tetrapyrrole ?
-
 ?
porphobilinogen Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
5 forms, A: native enzyme, B-E: isomeres corresponding to the enzyme-substrate intermediates Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity is temperature-dependent Homo sapiens
0.038
-
 A form Homo sapiens
0.039
-
 B form Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, forms A to E, stable for up to 18 months in 10 mM potassium phosphate buffer, pH 8.0, containing 0.2 mM dithioerythritol Homo sapiens
erythrocytes lysates stored in 1 mM potassium phosphate, pH 7.6, containing 0.05% Trition X-100, 1 mM dithiothreitol and 1 mM MgCl2, no or little loss of activity after 1 year Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Homo sapiens uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Homo sapiens hydroxylmethylbilane + 4 NH3
-
 ?
additional information occurrence of multiple forms of the enzyme. These isomers correspond to the enzyme-substrate intermediates: mono-, di-, tri-, and tetrapyrrole Homo sapiens ?
-
 ?
porphobilinogen
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE, A and B form Homo sapiens

Synonyms

Synonyms Comment Organism
uroporphyrinogen I synthase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Homo sapiens
56
-
 2h, no loss of activity Homo sapiens
60
-
 2 h, 10% loss of activity Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 porphobilinogen
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 A and B form Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: no activity below, pH 9.0: 50% of maximal activity Homo sapiens