Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
L116K | the specific activity of this recombinant mutant enzyme is 5fold higher than that of the recombinant wild type enzyme, catalyzes the formation of uroporphyrinogen I in addition to uroporphyrinogen III | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
porphobilinogen | recombinant wild type enzyme, 50 mM Tris, pH 7.5 containing 40 mM KCl, 0.8% Triton, 8% glycerol, and 2 mM beta-mercaptoethanol, at 37°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Plasmodium falciparum | 20011 | - |
membrane | - |
Plasmodium falciparum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
the purified protein appears as a doublet on SDS gel and the faint upper 45 kDa band may be because of the presence of the uncleaved periplasmic localization signal | Plasmodium falciparum |
45000 | - |
the purified protein appears as a doublet on SDS gel and the faint upper 45 kDa band may be because of the presence of the uncleaved periplasmic localization signal | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | A8CBH8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA resin chromatography | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen | - |
Plasmodium falciparum | uroporphyrinogen III + 4 NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PBGD | - |
Plasmodium falciparum |
porphobilinogen deaminase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | the enzyme loses very little activity even when heated to 80°C and then assayed at 37°C | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.5 | - |
Plasmodium falciparum |