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Literature summary for 2.5.1.61 extracted from
- Unique properties of Plasmodium falciparum porphobilinogen deaminase (2008), J. Biol. Chem., 283, 437-444.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L116K | the specific activity of this recombinant mutant enzyme is 5fold higher than that of the recombinant wild type enzyme, catalyzes the formation of uroporphyrinogen I in addition to uroporphyrinogen III | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
porphobilinogen | recombinant wild type enzyme, 50 mM Tris, pH 7.5 containing 40 mM KCl, 0.8% Triton, 8% glycerol, and 2 mM beta-mercaptoethanol, at 37°C | Plasmodium falciparum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| apicoplast | - |
Plasmodium falciparum | 20011 | - |
| membrane | - |
Plasmodium falciparum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
the purified protein appears as a doublet on SDS gel and the faint upper 45 kDa band may be because of the presence of the uncleaved periplasmic localization signal | Plasmodium falciparum |
| 45000 | - |
the purified protein appears as a doublet on SDS gel and the faint upper 45 kDa band may be because of the presence of the uncleaved periplasmic localization signal | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | A8CBH8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA resin chromatography | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | - |
Plasmodium falciparum | uroporphyrinogen III + 4 NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PBGD | - |
Plasmodium falciparum |
| porphobilinogen deaminase | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Plasmodium falciparum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 80 | the enzyme loses very little activity even when heated to 80°C and then assayed at 37°C | Plasmodium falciparum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.5 | - |
Plasmodium falciparum |
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Release 2023.1 (January 2023)
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