Cloned (Comment) | Organism |
---|---|
PCR-amplification, expression in Escherichia coli BL21Gold(DE3)(pLysS) with plasmid pET-14b(+)hemC | Desulfovibrio vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.214 | - |
porphobilinogen | 50 mM Tris-HCl, pH 8.0, 25°C | Desulfovibrio vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Desulfovibrio vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | Desulfovibrio vulgaris | - |
hydroxymethylbilane + 4 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | - |
sulfate-reducing bacterium | - |
Purification (Comment) | Organism |
---|---|
cells harvested and resuspended in 20 mM Tris-HCl buffer, pH 8.0 containing 500 mM NaCl and 10 mM imidazole, cells are lysed by sonication and centrifuged, applied to NiCl2 Sepahrose resin and eluted with buffer and 500 mM imidazole, pooled, concentrated by ultra centrifugation (10 kDa), buffer exchanged with 50 mM Tris-HCl, pH 8.0, with a PD10 column | Desulfovibrio vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
linked assay with HemB, CobA/HemD enzymes in 50 mM Tris-HCl, pH 8.0, 2 hours at 25°C produces precorrin-2 from the substrate aminolaevulinic acid | Desulfovibrio vulgaris |
0.333 | - |
200 nM porphobilinogen, 50 mM Tris-HCl, pH 8.0, 25°C | Desulfovibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen + H2O | - |
Desulfovibrio vulgaris | hydroxymethylbilane + 4 NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HemC | - |
Desulfovibrio vulgaris |
PBG deaminase | - |
Desulfovibrio vulgaris |
porphobilinogen deaminase | - |
Desulfovibrio vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dipyrromethane | in active site as shown by using Ehrlich's reagent | Desulfovibrio vulgaris |