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Literature summary for 2.5.1.61 extracted from

  • Lobo, S.A.; Brindley, A.; Warren, M.J.; Saraiva, L.M.
    Functional characterization of the early steps of tetrapyrrole biosynthesis and modification in Desulfovibrio vulgaris Hildenborough (2009), Biochem. J., 420, 317-325.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification, expression in Escherichia coli BL21Gold(DE3)(pLysS) with plasmid pET-14b(+)hemC Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.214
-
porphobilinogen 50 mM Tris-HCl, pH 8.0, 25°C Desulfovibrio vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
SDS-PAGE Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 porphobilinogen + H2O Desulfovibrio vulgaris
-
hydroxymethylbilane + 4 NH3
-
?

Organism

Organism UniProt Comment Textmining
Desulfovibrio vulgaris
-
sulfate-reducing bacterium
-

Purification (Commentary)

Purification (Comment) Organism
cells harvested and resuspended in 20 mM Tris-HCl buffer, pH 8.0 containing 500 mM NaCl and 10 mM imidazole, cells are lysed by sonication and centrifuged, applied to NiCl2 Sepahrose resin and eluted with buffer and 500 mM imidazole, pooled, concentrated by ultra centrifugation (10 kDa), buffer exchanged with 50 mM Tris-HCl, pH 8.0, with a PD10 column Desulfovibrio vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
linked assay with HemB, CobA/HemD enzymes in 50 mM Tris-HCl, pH 8.0, 2 hours at 25°C produces precorrin-2 from the substrate aminolaevulinic acid Desulfovibrio vulgaris
0.333
-
200 nM porphobilinogen, 50 mM Tris-HCl, pH 8.0, 25°C Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen + H2O
-
Desulfovibrio vulgaris hydroxymethylbilane + 4 NH3
-
?

Synonyms

Synonyms Comment Organism
HemC
-
Desulfovibrio vulgaris
PBG deaminase
-
Desulfovibrio vulgaris
porphobilinogen deaminase
-
Desulfovibrio vulgaris

Cofactor

Cofactor Comment Organism Structure
dipyrromethane in active site as shown by using Ehrlich's reagent Desulfovibrio vulgaris