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Literature summary for 2.5.1.62 extracted from

  • Shalygo, N.; Czarnecki, O.; Peter, E.; Grimm, B.
    Expression of chlorophyll synthase is also involved in feedback-control of chlorophyll biosynthesis (2009), Plant Mol. Biol., 71, 425-436.
    View publication on PubMed
Search for more literature for 2.5.1.62

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in the pGEM-T vector, cloning in the pBirAR vector for overexpression or gene silencing, transformation of Nicotiana tabacum leaf discs mediated by Agrobacterium tumefaciens strain GV2260 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Nicotiana tabacum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 calculated Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide a + geranylgeranyl diphosphate Nicotiana tabacum
-
 geranylgeranylchlorophyllide a + diphosphate
-
 ?
chlorophyllide a + phytyl diphosphate Nicotiana tabacum
-
 chlorophyll a + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum C3W4Q1 Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants
-
Nicotiana tabacum C3W4Q2 Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 leaf segments are laid out on buffer-moistened filter paper containing Zn chlorophyllide a for 8 h in the dark, formed product is analyzed by HPLC, overexpression leads to 120-140% chlorophyll synthase activity, expression of the antisense RNA reduces activity to less than 20% and 40% Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + geranylgeranyl diphosphate
-
 Nicotiana tabacum geranylgeranylchlorophyllide a + diphosphate
-
 ?
chlorophyllide a + phytyl diphosphate
-
 Nicotiana tabacum chlorophyll a + diphosphate
-
 ?
Zn chlorophyllide a + geranylgeranyl diphosphate
-
 Nicotiana tabacum Zn chlorophyll a + diphosphate
-
 ?
Zn chlorophyllide a + phytyl diphosphate
-
 Nicotiana tabacum Zn chlorophyll a + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
chlorophyll synthase
-
 Nicotiana tabacum
NtCHLG
-
 Nicotiana tabacum

General Information

General Information Comment Organism
malfunction reduced enzyme activity does not result in accumulation of chlorophyllide, it causes reduced 5-aminolevulinic acid formation and Mg chelatase and ferrochelatase activity, growth is retarded, leaves are pale green (20% wild type chlorophyll content), overexpression is correlated with enhanced 5-aminolevulinic acid synthesizing capacity and more chelatase activity, the results point to a feedback control of the biosynthesis pathway, the phenotype is not distinguishable from the wild type Nicotiana tabacum
metabolism enzyme catalyzes the last step in the chlorophyll biosynthetic pathway Nicotiana tabacum